Leica CM3050 S Thermoblock, Inserting specimen discs into the specimen head, Specimen orientation

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5.Operation

Thermoblock

The thermoblock makes it easy to remove fro- zen specimens from the specimen discs.

Do not store the thermoblock inside the cryochamber - store at room temperature and only place into the chamber for removing specimens from discs.

•Place cap (9) on one of the two ends so that the correct size opening (7) for the disc to be inserted is exposed.

•Insert pin (6) of specimen disc (5) into the opening (7).

•After about 20 seconds remove the frozen specimen from the specimen disc (use tweezers).

•If cap (9) fits too loosely, readjust with screw (10). Do not tighten screw too much!

•Once the specimen has been detached from the disc, remove the thermoblock from the cryochamber.

5.5.4Inserting specimen discs into the specimen head

• Lock handwheel in upper position.

If a knife has already been inserted into the knife holder, cover knife edge with knife guard!

•Loosen clamping screw (3).

•Insert specimen disc in opening (2) (rear surface of specimen disc must fit tightly against surface of specimen head).

•Tighten clamping screw (3).

5.5.5 Specimen orientation

•Open clamping lever (4) --> (4a).

•Orient specimen via orienting screws (1) and (5).

•Retighten clamping lever (4).

42	Instruction Manual V1.2 - 11/2006

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Symbols used in this manual and their meaning Important informationSerial- number Qualification of personnel GeneralDesignated use Any other use of the instrument is considered improperIntegrated safety devices SicherheitLocking SafetyLocking the handwheel UnlockingActivating the emergency stop function Deactivating the emergency stopKnife guard Site requirements Electrical connectionsHandling microtome knives/blades Defrosting/Handling frozen tissueFrozen parts of the instrument and frozen accessories Knife guard/handwheel lockDisinfection and cleaning Infectious/radioactive material11Removing/reinstalling the microtome MaintenanceDisplay message ‘Dry microtome’ General site requirements InstallationUnpacking and installation RepackingStandard delivery Further accessories Inserting the accessories Connecting the footswitch Footswitch FunctionModels with footswitch Prior to switching on the instrumentInstrument Properties OverviewInstrument Properties Technical Data Microtome Rotary microtome Operation Operating the instrument for the first timeMains switch / automatic cutout for the instrument Switching on/fusesSwitching on Automatic cutout for sectioning motorLock / unlock key Control panel Key functions in control panelDisplay functions in control panel Activates / deactivates a number of functionsY M I C R O T O M E Specimen temperature Instruments with specimen cooling only 15-minute stepsPress key until menu item ‘Preset counter’ is displayed Cryochamber temperaturePreset counter Instruments with sectioning motor only = Preset counter activatedDuration of defrost cycle Real timeStarting time of automatic defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStatus display StopThese parameters can be displayed via the ar- row keys Refrigeration stateSection thickness Total section thickness counter Control panel Instruments without sectioning motor Instruments with sectioning motorActivating a sectioning mode Sectioning mode 1 Intermittent strokeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Functional area B all models Coarse feed keys Coarse feed ‘fast forward’ Travelling speed 1,000 μm/sTo start, press and hold Setting the section thickness Selecting trimming thicknessMotorized trimming ‘Defrosting Handling frozen tissue’ Chapter Daily operation of the instrument‘Knife guard / Handwheel lock’ Chapter Mobile heat extractor Installing the 90 prismFreezing specimens with the 90 prism See .3.4 ‘Menu functions’ itemThermoblock Inserting specimen discs into the specimen headSpecimen orientation Sectioning Trimming / sectioning Inserting the knifeManual trimming Insert the knife / disposable blade into the knife holderSwitching off the instrument for a longer period of time Finishing work 5.6.1 Daily routine stepsLock handwheel Defrosting Automatic chamber defrost cycleInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Potential problems causes and remedies Problem Causes RemediesProblem Causes Luke-warm specimen disc Problem Causes Remedies Microtome defectiveReference chart for temperature settings in minus C TissueSpray disinfection with Leica Cryofect Disinfection, cleaning and maintenanceSafety instructions On disinfection and cleaning Allow to soak for 15 minutesDisinfection with conventional disinfectants CleaningSwitch the instrument off Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Reinstalling the microtome Place plastic coupling 11 on handwheel couplingUnlock handwheel Removal Installing the replacement lampExchanging the fluorescent light lamp Removing the sliding windowTechnical service information Warranty and serviceWarranty Decommissioning and disposalElectrohydraulic height adjustment Safety instructionsAppendix Installation Maintenance and care TroubleshootingControl keys Specimen discs Ordering information accessories Knife holdersAccessories ConsumablesEC Declaration of Conformity Dag Graupner Managing DirectorDear Customer This equipment has been cleaned and decontaminatedDecontamination Certificate master NB Microtome knives must be in boxesBU Return Authorisation Number