Leica CM3050 S Installation, General site requirements, Unpacking and installation, Repacking

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3.Installation

3.1Site requirements

Make sure to read and follow all safety instructions in chapter 2.3.2 ‘Site requirements’!

3.1.1 General site requirements

•No direct sunlight.

•Electrical power supply within distance (length of power cord = approx. 4 meters - do not use extension cords!).

•No draft (caused by air conditioning etc.).

•Even floor surface.

•Practically vibration-free floor.

•Handwheel easily accessible.

•Room temperature constantly below +22 °C.

•Relative humidity of air maximum 60 %.

High ambient temperature and/or high air humidity negatively affect instrument cooling performance!

3.1.2 Electrical connections

Make sure to read and follow all safety instructions in chapter 2.3.3 ‘Electrical connections’!

3.2Unpacking and installation

Unpacking instructions are always located in a transparent protective envelope on the outside of the instrument shipping crate.

Make sure to read and follow all safety instructions provided in chapter 2.3.1 ‘Transport’ and on the unpacking instructions!

3.2.1 Repacking

We recommend to keep the original shipping crate and the unpacking instructions for the Leica CM3050 S.

For repacking, proceed as per unpacking in- structions, in reverse order.

Available models

•Basic instrument

-with sectioning motor

-without specimen cooling

•Basic instrument

-without sectioning motor

-with specimen cooling

•Basic instrument

-with sectioning motor

-with specimen cooling

16	Instruction Manual V1.2 - 11/2006

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Important information Symbols used in this manual and their meaningSerial- number General Designated useQualification of personnel Any other use of the instrument is considered improperIntegrated safety devices SicherheitSafety Locking the handwheelLocking UnlockingDeactivating the emergency stop Activating the emergency stop functionKnife guard Site requirements Electrical connectionsDefrosting/Handling frozen tissue Frozen parts of the instrument and frozen accessoriesHandling microtome knives/blades Knife guard/handwheel lockDisinfection and cleaning Infectious/radioactive materialMaintenance 11Removing/reinstalling the microtomeDisplay message ‘Dry microtome’ Installation Unpacking and installationGeneral site requirements RepackingStandard delivery Further accessories Inserting the accessories Footswitch Function Models with footswitchConnecting the footswitch Prior to switching on the instrumentInstrument Properties OverviewInstrument Properties Technical Data Microtome Rotary microtome Operation Operating the instrument for the first timeSwitching on/fuses Switching onMains switch / automatic cutout for the instrument Automatic cutout for sectioning motorControl panel Key functions in control panel Display functions in control panelLock / unlock key Activates / deactivates a number of functionsY M I C R O T O M E Specimen temperature Instruments with specimen cooling only 15-minute stepsCryochamber temperature Preset counter Instruments with sectioning motor onlyPress key until menu item ‘Preset counter’ is displayed = Preset counter activatedReal time Starting time of automatic defrost cycleDuration of defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStatus display StopRefrigeration state These parameters can be displayed via the ar- row keysSection thickness Total section thickness counter Control panel Instruments without sectioning motor Instruments with sectioning motorSectioning mode 1 Intermittent stroke Activating a sectioning modeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Coarse feed ‘fast forward’ Travelling speed 1,000 μm/s Functional area B all models Coarse feed keysTo start, press and hold Selecting trimming thickness Setting the section thicknessMotorized trimming Daily operation of the instrument ‘Defrosting Handling frozen tissue’ Chapter‘Knife guard / Handwheel lock’ Chapter Installing the 90 prism Freezing specimens with the 90 prismMobile heat extractor See .3.4 ‘Menu functions’ itemInserting specimen discs into the specimen head ThermoblockSpecimen orientation Trimming / sectioning Inserting the knife Manual trimmingSectioning Insert the knife / disposable blade into the knife holderFinishing work 5.6.1 Daily routine steps Switching off the instrument for a longer period of timeLock handwheel Automatic chamber defrost cycle DefrostingInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Potential problems causes and remedies Problem Causes RemediesProblem Causes Luke-warm specimen disc Problem Causes Remedies Microtome defectiveReference chart for temperature settings in minus C TissueDisinfection, cleaning and maintenance Safety instructions On disinfection and cleaningSpray disinfection with Leica Cryofect Allow to soak for 15 minutesCleaning Switch the instrument offDisinfection with conventional disinfectants Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Place plastic coupling 11 on handwheel coupling Reinstalling the microtomeUnlock handwheel Installing the replacement lamp Exchanging the fluorescent light lampRemoval Removing the sliding windowWarranty and service WarrantyTechnical service information Decommissioning and disposalSafety instructions Electrohydraulic height adjustmentAppendix Installation Troubleshooting Maintenance and careControl keys Ordering information accessories Knife holders AccessoriesSpecimen discs ConsumablesEC Declaration of Conformity Dag Graupner Managing DirectorThis equipment has been cleaned and decontaminated Decontamination Certificate masterDear Customer NB Microtome knives must be in boxesBU Return Authorisation Number