Leica CM3050 S instruction manual Table of Contents

Page 5

 

 

Table of Contents

 

 

 

 

 

 

4.

Instrument Properties

21

 

4.1

Overview

21

 

4.2

Technical Data

23

5.

Operation

25

 

5.1

Operating the instrument for the first time

25

 

5.2

Switching on/fuses

26

 

5.3

Control panel 1

27

 

5.3.1

Key functions in control panel 1

27

 

5.3.2

Display functions in control panel 1

27

 

5.3.3

Display indications when switching on

28

 

5.3.4

Menu functions: setting parameters of refrigeration, time and preset counter

29

 

5.3.5

Status display

32

 

5.3.6

Indication of refrigeration state, section thickness setting and counters

33

 

5.4

Control panel 2

34

 

5.4.1

Selecting a sectioning mode /

35

 

5.4.2

Setting the sectioning window

36

 

5.4.3

Selecting sectioning speed

37

 

5.4.4

Emergency stop

37

 

5.4.5

Coarse feed keys

38

 

5.4.6

Trimming and sectioning functions

39

 

5.4.7

Setting the section thickness

39

 

5.5

Daily operation of the instrument

40

 

5.5.1

Freezing specimens onto specimen

40

 

5.5.2

Freezing specimens onto specimen

41

 

5.5.3

Optional accessories for freezing

41

 

5.5.4

Inserting specimen discs into the

42

 

5.5.5

Specimen orientation

42

 

5.5.6

Trimming / sectioning

43

 

5.6

Finishing work

44

 

5.6.1

Daily routine steps

44

 

5.6.2

Switching off the instrument for a

44

 

5.7

Defrosting

45

 

5.7.1

Automatic chamber defrost cycle

45

 

5.7.2

Manual chamber defrost cycle

46

 

5.7.3 Defrosting the specimen head

46

Leica CM3050S – Cryostat

5

Image 5
Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Serial- number Symbols used in this manual and their meaningImportant information Designated use GeneralQualification of personnel Any other use of the instrument is considered improperSicherheit Integrated safety devicesLocking the handwheel SafetyLocking UnlockingKnife guard Activating the emergency stop functionDeactivating the emergency stop Electrical connections Site requirementsFrozen parts of the instrument and frozen accessories Defrosting/Handling frozen tissueHandling microtome knives/blades Knife guard/handwheel lockInfectious/radioactive material Disinfection and cleaningDisplay message ‘Dry microtome’ 11Removing/reinstalling the microtomeMaintenance Unpacking and installation InstallationGeneral site requirements RepackingStandard delivery Further accessories Inserting the accessories Models with footswitch Footswitch FunctionConnecting the footswitch Prior to switching on the instrumentOverview Instrument PropertiesInstrument Properties Technical Data Microtome Rotary microtome Operating the instrument for the first time OperationSwitching on Switching on/fusesMains switch / automatic cutout for the instrument Automatic cutout for sectioning motorDisplay functions in control panel Control panel Key functions in control panelLock / unlock key Activates / deactivates a number of functionsY M I C R O T O M E 15-minute steps Specimen temperature Instruments with specimen cooling onlyPreset counter Instruments with sectioning motor only Cryochamber temperaturePress key until menu item ‘Preset counter’ is displayed = Preset counter activatedStarting time of automatic defrost cycle Real timeDuration of defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStop Status displaySection thickness Total section thickness counter These parameters can be displayed via the ar- row keysRefrigeration state Instruments with sectioning motor Control panel Instruments without sectioning motorStart / stop motorized sectioning Activating a sectioning modeSectioning mode 1 Intermittent stroke Press sectioning window key once more Emergency stop To start, press and hold Functional area B all models Coarse feed keysCoarse feed ‘fast forward’ Travelling speed 1,000 μm/s Motorized trimming Setting the section thicknessSelecting trimming thickness ‘Knife guard / Handwheel lock’ Chapter ‘Defrosting Handling frozen tissue’ ChapterDaily operation of the instrument Freezing specimens with the 90 prism Installing the 90 prismMobile heat extractor See .3.4 ‘Menu functions’ itemSpecimen orientation ThermoblockInserting specimen discs into the specimen head Manual trimming Trimming / sectioning Inserting the knifeSectioning Insert the knife / disposable blade into the knife holderLock handwheel Switching off the instrument for a longer period of timeFinishing work 5.6.1 Daily routine steps Instruments with specimen cooling DefrostingAutomatic chamber defrost cycle Manual chamber defrost cycle Troubleshooting, applications tips Remedies Potential problems causes and remedies Problem CausesProblem Causes Luke-warm specimen disc Microtome defective Problem Causes RemediesTissue Reference chart for temperature settings in minus CSafety instructions On disinfection and cleaning Disinfection, cleaning and maintenanceSpray disinfection with Leica Cryofect Allow to soak for 15 minutesSwitch the instrument off CleaningDisinfection with conventional disinfectants Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Unlock handwheel Reinstalling the microtomePlace plastic coupling 11 on handwheel coupling Exchanging the fluorescent light lamp Installing the replacement lampRemoval Removing the sliding windowWarranty Warranty and serviceTechnical service information Decommissioning and disposalAppendix Electrohydraulic height adjustmentSafety instructions Installation Control keys Maintenance and careTroubleshooting Accessories Ordering information accessories Knife holdersSpecimen discs ConsumablesDag Graupner Managing Director EC Declaration of ConformityDecontamination Certificate master This equipment has been cleaned and decontaminatedDear Customer NB Microtome knives must be in boxesBU Return Authorisation Number