Leica CM3050 S instruction manual Operation, Operating the instrument for the first time

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5.Operation

5.1 Operating the instrument for the first time

Prior to setting up the instrument, make sure to read the instruction manual carefully and in full.

When setting up the instrument, please follow the individual chapters in the following order:

First, study chapters 1, 2, and 3 - install the instrument as described in chapter 3.

The overview in chapter 4.1 is helpful during the unpacking and instal- lation procedure in order to identify individual parts and place them in the correct location.

Go on to chapter 5.

Read chapters 5.1, 5.2, 5.3 and 5.4 to learn all about the controls.

Next, start to work with the instrument (chapter 5.5). Follow the instruc- tions in chapter 5.5.

Chapter 5.5.2 applies to instruments with specimen cooling only.

The temperature chart in chapter 6.3 is useful when it comes to select- ing appropriate sectioning temperatures for different types of speci- mens.

When disinfecting / cleaning the instrument and/or removing / rein- stalling the microtome, please follow the instructions in chapter 7.

In case of problems and malfunctions, please refer to chapters 6.1 and 6.2. These chapters list the most common types of problems which many times have simple causes that can be eliminated by the actual user.

If you find ‘Call Technical Service’ in the ‘Remedy’ column, please do so. Never try to solve these problems yourself!

Leica CM3050 S – Cryostat

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Important information Symbols used in this manual and their meaningSerial- number Designated use GeneralQualification of personnel Any other use of the instrument is considered improperSicherheit Integrated safety devicesLocking the handwheel SafetyLocking UnlockingDeactivating the emergency stop Activating the emergency stop functionKnife guard Electrical connections Site requirementsFrozen parts of the instrument and frozen accessories Defrosting/Handling frozen tissueHandling microtome knives/blades Knife guard/handwheel lockInfectious/radioactive material Disinfection and cleaningMaintenance 11Removing/reinstalling the microtomeDisplay message ‘Dry microtome’ Unpacking and installation InstallationGeneral site requirements RepackingStandard delivery Further accessories Inserting the accessories Models with footswitch Footswitch FunctionConnecting the footswitch Prior to switching on the instrumentOverview Instrument PropertiesInstrument Properties Technical Data Microtome Rotary microtome Operating the instrument for the first time OperationSwitching on Switching on/fusesMains switch / automatic cutout for the instrument Automatic cutout for sectioning motorDisplay functions in control panel Control panel Key functions in control panelLock / unlock key Activates / deactivates a number of functionsY M I C R O T O M E 15-minute steps Specimen temperature Instruments with specimen cooling onlyPreset counter Instruments with sectioning motor only Cryochamber temperaturePress key until menu item ‘Preset counter’ is displayed = Preset counter activatedStarting time of automatic defrost cycle Real timeDuration of defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStop Status displayRefrigeration state These parameters can be displayed via the ar- row keysSection thickness Total section thickness counter Instruments with sectioning motor Control panel Instruments without sectioning motorSectioning mode 1 Intermittent stroke Activating a sectioning modeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Coarse feed ‘fast forward’ Travelling speed 1,000 μm/s Functional area B all models Coarse feed keysTo start, press and hold Selecting trimming thickness Setting the section thicknessMotorized trimming Daily operation of the instrument ‘Defrosting Handling frozen tissue’ Chapter‘Knife guard / Handwheel lock’ Chapter Freezing specimens with the 90 prism Installing the 90 prismMobile heat extractor See .3.4 ‘Menu functions’ itemInserting specimen discs into the specimen head ThermoblockSpecimen orientation Manual trimming Trimming / sectioning Inserting the knifeSectioning Insert the knife / disposable blade into the knife holderFinishing work 5.6.1 Daily routine steps Switching off the instrument for a longer period of timeLock handwheel Automatic chamber defrost cycle DefrostingInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Remedies Potential problems causes and remedies Problem CausesProblem Causes Luke-warm specimen disc Microtome defective Problem Causes RemediesTissue Reference chart for temperature settings in minus CSafety instructions On disinfection and cleaning Disinfection, cleaning and maintenanceSpray disinfection with Leica Cryofect Allow to soak for 15 minutesSwitch the instrument off CleaningDisinfection with conventional disinfectants Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Place plastic coupling 11 on handwheel coupling Reinstalling the microtomeUnlock handwheel Exchanging the fluorescent light lamp Installing the replacement lampRemoval Removing the sliding windowWarranty Warranty and serviceTechnical service information Decommissioning and disposalSafety instructions Electrohydraulic height adjustmentAppendix Installation Troubleshooting Maintenance and careControl keys Accessories Ordering information accessories Knife holdersSpecimen discs ConsumablesDag Graupner Managing Director EC Declaration of ConformityDecontamination Certificate master This equipment has been cleaned and decontaminatedDear Customer NB Microtome knives must be in boxesBU Return Authorisation Number