Leica CM3050 S instruction manual Technical Data

Page 23

4.Instrument properties

4.2 Technical Data

Operating temperature range (ambient temperature): + 18°C to + 40°C. All specifications related to tempe- rature are valid only up to an ambient temperature of + 22°C and relative air humidity of less than 60%!

Type

CM3050S-10

CM3050S-1

CM3050S-8

CM3050S-9

Marks of conformity

-

-

-

-

 

Nominal voltage

100 V AC ±10%

230 V AC ±10%

240 V AC ±10% 100 V AC ±10%

Nominal frequency

50 Hz

50 Hz

50 Hz

60 Hz

Power draw

1800 VA

1800 VA

1800 VA

1800 VA

Max. start-up current for 5 sec

35 A eff.

25 A eff.

25 A eff.

30 A eff.

Protective fuse

I

I

I

I

Automatic cutout

T15A M3

T10A T1

T10A T1

T15A M3

Pollution degree￿

2

2

2

2

 

Overvoltage installation category

II

II

II

II

Heat emission (max.)

1800 J/s

1800 J/s

1800 J/s

1800 J/s

 

 

 

 

 

 

Refrigeration system

50 Hz

 

 

60 Hz

Cryochamber

 

 

 

 

 

Temperature selection range

0° C to -40°C (+ 2 K / - 0 K)

 

0° C to -40°C (+2 K / - 0 K)

Defrosting

Automatic hot-gas defrost cycle,

Automatic hot-gas defrost cycle,

 

programmable in 15-min increments;

programmable in 15-min. increments;

 

1 automatic defrost cycle per 24 hours.

1 automatic defrost cycle per 24 hours.

 

Duration: 6 - 12 minutes

 

Duration: 6 - 12 minutes

Refrigeration capacity￿

Manual defrost cycle

 

Manual defrost cycle

690 W

 

 

690 W

Safety factor

3

 

 

3

 

Refrigerant

280 g ±5g refrigerant R 404A *

 

280 g ±5g refrigerant R 404A*

Compressor oil

0.6 l EMKARATE RL-22S, ICI *

 

0.6 l EMKARATE RL-22S, ICI *

Quick-freeze shelf

 

 

 

 

 

Maximum temperature

- 43°C (+ 0 K / - 2 K)

 

- 43°C (+ 0 K / - 2 K)

Number of quick-freeze stations

10

 

 

10

 

Specimen cooling (Option)

 

 

 

 

 

Temperature range

-10° C to -50°C ±3K

 

-10° C to -50°C ±3K

Defrosting

Manual defrost cycle (electric)

 

Manual defrost cycle (electric)

Refrigeration capacity￿

320 W

 

 

320 W

Safety factor

3

 

 

3

 

Refrigerant

185 g ±5g refrigerant R 404A *

 

185 g ±5g refrigerant R 404A *

Compressor oil

0.4 l alpha 22, Kyodo *

 

0.4 l alpha 22, Kyodo *

 

or RENISO E22, Fuchs *

 

or RENISO E22, Fuchs *

 

 

 

 

 

 

 

 

 

 

 

 

Leica CM3050S – Cryostat

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Serial- number Symbols used in this manual and their meaningImportant information Any other use of the instrument is considered improper GeneralDesignated use Qualification of personnelSicherheit Integrated safety devicesUnlocking SafetyLocking the handwheel LockingKnife guard Activating the emergency stop functionDeactivating the emergency stop Electrical connections Site requirementsKnife guard/handwheel lock Defrosting/Handling frozen tissueFrozen parts of the instrument and frozen accessories Handling microtome knives/bladesInfectious/radioactive material Disinfection and cleaningDisplay message ‘Dry microtome’ 11Removing/reinstalling the microtomeMaintenance Repacking InstallationUnpacking and installation General site requirementsStandard delivery Further accessories Inserting the accessories Prior to switching on the instrument Footswitch FunctionModels with footswitch Connecting the footswitchOverview Instrument PropertiesInstrument Properties Technical Data Microtome Rotary microtome Operating the instrument for the first time OperationAutomatic cutout for sectioning motor Switching on/fusesSwitching on Mains switch / automatic cutout for the instrumentActivates / deactivates a number of functions Control panel Key functions in control panelDisplay functions in control panel Lock / unlock keyY M I C R O T O M E 15-minute steps Specimen temperature Instruments with specimen cooling only= Preset counter activated Cryochamber temperaturePreset counter Instruments with sectioning motor only Press key until menu item ‘Preset counter’ is displayedPress lock/unlock key to activate or de- activate ON/OFF Real timeStarting time of automatic defrost cycle Duration of defrost cycleStop Status displaySection thickness Total section thickness counter These parameters can be displayed via the ar- row keysRefrigeration state Instruments with sectioning motor Control panel Instruments without sectioning motorStart / stop motorized sectioning Activating a sectioning modeSectioning mode 1 Intermittent stroke Press sectioning window key once more Emergency stop To start, press and hold Functional area B all models Coarse feed keysCoarse feed ‘fast forward’ Travelling speed 1,000 μm/s Motorized trimming Setting the section thicknessSelecting trimming thickness ‘Knife guard / Handwheel lock’ Chapter ‘Defrosting Handling frozen tissue’ ChapterDaily operation of the instrument See .3.4 ‘Menu functions’ item Installing the 90 prismFreezing specimens with the 90 prism Mobile heat extractorSpecimen orientation ThermoblockInserting specimen discs into the specimen head Insert the knife / disposable blade into the knife holder Trimming / sectioning Inserting the knifeManual trimming SectioningLock handwheel Switching off the instrument for a longer period of timeFinishing work 5.6.1 Daily routine steps Instruments with specimen cooling DefrostingAutomatic chamber defrost cycle Manual chamber defrost cycle Troubleshooting, applications tips Remedies Potential problems causes and remedies Problem CausesProblem Causes Luke-warm specimen disc Microtome defective Problem Causes RemediesTissue Reference chart for temperature settings in minus CAllow to soak for 15 minutes Disinfection, cleaning and maintenanceSafety instructions On disinfection and cleaning Spray disinfection with Leica CryofectRemove section waste from the chamber and dispose of it CleaningSwitch the instrument off Disinfection with conventional disinfectants‘Disinfection and cleaning’ Chapter Unlock handwheel Reinstalling the microtomePlace plastic coupling 11 on handwheel coupling Removing the sliding window Installing the replacement lampExchanging the fluorescent light lamp RemovalDecommissioning and disposal Warranty and serviceWarranty Technical service informationAppendix Electrohydraulic height adjustmentSafety instructions Installation Control keys Maintenance and careTroubleshooting Consumables Ordering information accessories Knife holdersAccessories Specimen discsDag Graupner Managing Director EC Declaration of ConformityNB Microtome knives must be in boxes This equipment has been cleaned and decontaminatedDecontamination Certificate master Dear CustomerBU Return Authorisation Number