Leica CM1950 manual Safety Features, Microtome knives

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2.Safety and Design

2.3Safety Features

The instruction manual includes important information related to the operating safety and maintenance of the instrument.

The Operating Manual is an important part of the product, and must be read carefully before startup and use and must always be kept near the instrument.

If additional requirements on accident prevention and environmental protection apply in the country of operation, this Operating Manual must be supplemented by appropriate instructions to ensure compliance with such requirements.

The instrument is equipped with the following safety devices: an emergen- cy stop switch (motorized instruments only), a handwheel lock and center- ing system (motorized instruments only), knife guard on the blade and knife holder, and a blade ejector.

To prevent adverse health effects from UV radiation, the UV disinfec- tion cycle can be started only after the sliding window has been prop- erly closed. Closing the window activates the corresponding safety features.

The consistent use of these safety features and strict observation of the warn- ings and cautions in this manual, will safeguard the operator from accidents and/or personal injury to a great extent.

Microtome knives

Take care when handling microtome knives/disposable blades. The cutting edge is extremely sharp and can cause severe injury!

Never leave knives and dismounted knife holders with a knife/blade mounted lying around!

Do not place a knife on a table with the cutting edge facing upwards!

We strongly recommend using the safety gloves included with the standard delivery!

Never try to catch a falling knife!

Prior to manipulating the knife and specimen or changing the specimen, always lock the handwheel and cover the cutting edge with the knife guard!

Avoid contact with cold parts of the instrument as this can cause frostbite!

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Operating Manual V1.1 – 05/2007

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Contents Leica CM1950 Page Page Table of contents 12.2 11.112.1 12.3Qualification of Personnel Symbols Used in This Manual and Their MeaningImportant Information Designated UseGeneral Safety Instructions Safety and DesignUnpacking and Installation Opening the packaging Transport to the Installation Location Ramp assemblyRemoving the packaging Safety Features Microtome knivesKnife guard Securing/Locking the HandwheelCleaning, Disinfection Turning the Instrument Back On Handling Specimens Defrosting MaintenanceRemoval of the Microtome Technical Data Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 HzStandard Delivery Set of filters 5 piecesHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Right side InstallationSite Requirements Left sideTransport to the Site Assembling the Handwheel Centering the Handwheel Optional Locking/Unlocking the HandwheelEmergency-stop function active, or Existing power system Connection to Power Supply SystemOnly for instruments sold in Japan Selecting the frequency Installation Inserting the Section Waste Tray Installing the Storage Systems Optional For reasonsShelf, Movable Optional Section waste tray with the a Cutout a facing the userInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Instrument Controls Control Panel Fields on the CM1950 Control Panel Field12 o’clock Position 6 o’clock 20.0μm Retraction0μm 60.0μmMotion continues as long as the button is held Coarse feed functionsBrief press of the button retracts the specimen by 20μm Control panel 3 Motorized sectioning optional Sectioning modesDisinfection Duration 30min Duration 180 min Leica Cryofect is not available in all countriesWorking with the Instrument Turning the Instrument OnConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Cutting with Extraction Anti-roll Guide installed Shut off VAC LED in VAC button goes out10μm 40% 60% 80% 100% 20μm 30μm 40μm SpeedSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Working with the Instrument Problems During Work TroubleshootingProblem Causes Remedies Cryochamber Sections tear Alternately Longer sufficient Temperature Selection Chart in minus C Tissue type Chamber temperatureOptional Accessories Ordering InformationCryofect, VPE Leica Cryofect 4x 350ml Lever 6 for the lateral shift When using low-profile blades, the rulerWith anti-roll guide Lever 7 for clamping the bladeBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Cleaning the knife holder CE Daily cleaning Unscrew the bolts 35 of the pressure plateNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories Maintenance and Cleaning General MaintenanceChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp NB Microtome knives must be in boxes This equipment has been cleaned and decontaminatedDecontamination Certificate master Yes No indicated belowBU Return Authorization Number Anne De Greef-Safft President Biosystems Division EC Declaration of ConformityWarranty and Service Peoples Republic of China