Leica CM1950 manual Entering the trimming thickness

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8.Working with the Instrument

Entering the trimming thickness

To switch from a trimming section thickness for research applica- tions (1-600 μm) to a thickness for clinical applications (10, 20, 30 or 40 μm), press and hold down the TRIM/SECT key while switching on the instrument.

Press the TRIM/SECT button. TRIM mode is active if the LED at the top right is illuminated.

Set the desired trimming thickness using the "+" or "-" button in control panel field 2. (Refer to page 32 for adjustable sequence of steps)

Entering the section thickness

Press the TRIM/SECT button. SECT mode is active if the LED at the bot- tom right is illuminated.

Set the desired section thickness using the "+" or "-" button in control panel field 2. (Refer to page 32 for adjustable sequence of steps)

Switching the retraction on or off in manual sectioning mode

Press the TRIM/SECT button for approx. 3 sec. The LED in control panel

field 2 displays on or oFF .

You can switch by pressing the "+" or "-" button.

"Retraction on" means a specimen retraction of 20 μm in manual mode.

During motorized sectioning, the retraction value is speed- dependent and cannot be changed by the user.

Leica CM1950 Cryostat

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Contents Leica CM1950 Page Page Table of contents 12.1 11.112.2 12.3Important Information Symbols Used in This Manual and Their MeaningQualification of Personnel Designated UseUnpacking and Installation Safety and DesignGeneral Safety Instructions Opening the packaging Removing the packaging Ramp assemblyTransport to the Installation Location Microtome knives Safety FeaturesSecuring/Locking the Handwheel Knife guardCleaning, Disinfection Turning the Instrument Back On Removal of the Microtome MaintenanceHandling Specimens Defrosting Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 Hz Technical DataSet of filters 5 pieces Standard DeliveryHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Site Requirements InstallationRight side Left sideTransport to the Site Assembling the Handwheel Emergency-stop function active, or Locking/Unlocking the HandwheelCentering the Handwheel Optional Only for instruments sold in Japan Selecting the frequency Connection to Power Supply SystemExisting power system Installation Shelf, Movable Optional Installing the Storage Systems Optional For reasonsInserting the Section Waste Tray Section waste tray with the a Cutout a facing the userInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Control Panel Fields on the CM1950 Control Panel Field Instrument Controls12 o’clock Position 6 o’clock 0μm Retraction20.0μm 60.0μmBrief press of the button retracts the specimen by 20μm Coarse feed functionsMotion continues as long as the button is held Sectioning modes Control panel 3 Motorized sectioning optionalLeica Cryofect is not available in all countries Disinfection Duration 30min Duration 180 minTurning the Instrument On Working with the InstrumentConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Shut off VAC LED in VAC button goes out Cutting with Extraction Anti-roll Guide installedSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Speed10μm 40% 60% 80% 100% 20μm 30μm 40μm Working with the Instrument Problem Causes Remedies TroubleshootingProblems During Work Cryochamber Sections tear Alternately Longer sufficient Tissue type Chamber temperature Temperature Selection Chart in minus COrdering Information Optional AccessoriesCryofect, VPE Leica Cryofect 4x 350ml With anti-roll guide When using low-profile blades, the rulerLever 6 for the lateral shift Lever 7 for clamping the bladeBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Unscrew the bolts 35 of the pressure plate Cleaning the knife holder CE Daily cleaningNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories General Maintenance Maintenance and CleaningChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp Decontamination Certificate master This equipment has been cleaned and decontaminatedNB Microtome knives must be in boxes Yes No indicated belowBU Return Authorization Number EC Declaration of Conformity Anne De Greef-Safft President Biosystems DivisionWarranty and Service Peoples Republic of China