Leica CM1950 manual Sections tear

9. Troubleshooting

Problem	Causes	Remedies
	Blunt knife.	Use different part of the knife.
	Inappropriate section thickness.	Select correct section thickness.
	Incorrect clearance angle.	Set correct angle.
	Microtome not dried thoroughly	Dry microtome.
	enough.
	Dried specimen.	Prepare new specimen.

Tissue sticks to the anti-roll plate

Anti-roll plate is too warm or incorrectly positioned.

Fat on the corner or edge of anti-roll plate.

Anti-roll plate not correctly fixed. Rust on the knife.

Cool down anti-roll plate, or reposition correctly.

Remove fat from anti-roll plate.

Fix correctly.

Remove rust.

Flattened sections curl up when anti-roll plate is folded up

Anti-roll plate too warm. Cool down anti-roll plate.

Sections tear	Temperature too low for the tissue cut.	Increase temperature and wait.
	Blunt part, dirt, dust, frost or rust on	Remove cause.
	the knife.
	Top edge of the anti-roll plate	Replace anti-roll plate.
	damaged.
	Hard particles in the tissue.	- - -
	Dirt on knife back.	Clean.

Cryostat non-operational	Power plug not properly connected.
	Defective fuses, or circuit breaker has
	triggered.

Check if properly connected. Replace fuses, or switch circuit breaker back on. If not possible, call technical service.

Specimen disc cannot be	Moisture on the underside cause the
removed	specimen to freeze to the freezing
	shelf or specimen head.

Apply concentrated alcohol to the contact point.

50 Operating Manual V1.1 – 05/2007