Leica CM1950 manual Working with the Instrument

Page 47

8. Working with the Instrument

After trimming or sectioning:

Specimen:

Unclamp and thaw.

Immerse in fixative for further processing.

Cleaning

• Use the brush to sweep up the section waste (section waste tray) and remove it from the cryostat (follow applicable laboratory regulations for disposal).

Or:

Clean the cryostat chamber using the chamber suction nozzle:

To do so, turn the (flat) suction nozzle of the extraction hose by holding the hose on the white adapter and removing it with a quick twist. "Park" the flat suction nozzle in a designated place in the chamber, e.g. on the right inside wall of the cryogenic chamber.

Remove the chamber suction nozzle from the plastic clip and attach it firmly to the white adapter.

Check the remaining capacity of the filter (in the interior of the chamber) and change the filter if necessary (see page 28).

Check the bacterial filter (in the front of the unit, see page 28), change it at least every 3 months.

Move the anti-roll guide to the side.

Spray the cryostat chamber using Cryofect.

Activate the UVC disinfection.

Leica CM1950 Cryostat

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Contents Leica CM1950 Page Page Table of contents 12.3 11.112.1 12.2Designated Use Symbols Used in This Manual and Their MeaningImportant Information Qualification of PersonnelUnpacking and Installation Safety and DesignGeneral Safety Instructions Opening the packaging Removing the packaging Ramp assemblyTransport to the Installation Location Microtome knives Safety FeaturesSecuring/Locking the Handwheel Knife guardCleaning, Disinfection Turning the Instrument Back On Removal of the Microtome MaintenanceHandling Specimens Defrosting Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 Hz Technical DataSet of filters 5 pieces Standard DeliveryHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Left side InstallationSite Requirements Right sideTransport to the Site Assembling the Handwheel Emergency-stop function active, or Locking/Unlocking the HandwheelCentering the Handwheel Optional Only for instruments sold in Japan Selecting the frequency Connection to Power Supply SystemExisting power system Installation Section waste tray with the a Cutout a facing the user Installing the Storage Systems Optional For reasonsShelf, Movable Optional Inserting the Section Waste TrayInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Control Panel Fields on the CM1950 Control Panel Field Instrument Controls12 o’clock Position 6 o’clock 60.0μm Retraction0μm 20.0μmBrief press of the button retracts the specimen by 20μm Coarse feed functionsMotion continues as long as the button is held Sectioning modes Control panel 3 Motorized sectioning optionalLeica Cryofect is not available in all countries Disinfection Duration 30min Duration 180 minTurning the Instrument On Working with the InstrumentConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Shut off VAC LED in VAC button goes out Cutting with Extraction Anti-roll Guide installedSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Speed10μm 40% 60% 80% 100% 20μm 30μm 40μm Working with the Instrument Problem Causes Remedies TroubleshootingProblems During Work Cryochamber Sections tear Alternately Longer sufficient Tissue type Chamber temperature Temperature Selection Chart in minus COrdering Information Optional AccessoriesCryofect, VPE Leica Cryofect 4x 350ml Lever 7 for clamping the blade When using low-profile blades, the rulerWith anti-roll guide Lever 6 for the lateral shiftBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Unscrew the bolts 35 of the pressure plate Cleaning the knife holder CE Daily cleaningNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories General Maintenance Maintenance and CleaningChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp Yes No indicated below This equipment has been cleaned and decontaminatedDecontamination Certificate master NB Microtome knives must be in boxesBU Return Authorization Number EC Declaration of Conformity Anne De Greef-Safft President Biosystems DivisionWarranty and Service Peoples Republic of China