Leica CM1950 manual Handling Specimens Defrosting, Removal of the Microtome, Maintenance

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2. Safety and Design

2.6Handling Specimens – Defrosting

•When working with contaminated or infected material, the general safety guidelines for laboratories must be applied.

•Before defrosting the cryochamber remove all samples!

•Before defrosting the specimen head, remove all samples!

Never leave samples in the cryochamber! - The instrument is not suitable for storing frozen specimens, as the refrigeration dehy- drates the specimens!

The quick freeze shelf can become very hot during the defrosting process. Do not touch it during defrosting.

2.7Removal of the Microtome

•The microtome is encapsulated and therefore does not require removal by the user.

2.8Maintenance

Replacing the fuses

•Turn the instrument off and disconnect the power plug before replacing the fuses.

•Use only the fuse types specified in chapter 3, "Technical Data." Failure to comply with this can cause severe damage to the instrument!

Replacing the fluorescent lamp/UVC lamp

•Turn the instrument off and disconnect the power plug before replacing the lamps.

It is possible to break the UVC lamp during replacement. If this happens, the lamp change must be completed by Technical Ser- vice. If any metallic mercury is released, handle it carefully and dispose of it properly.

If both disinfection indicator lights are blinking alternately, the

UV tube must be replaced!

Leica CM1950 Cryostat

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Contents Leica CM1950 Page Page Table of contents 12.1 11.112.2 12.3Important Information Symbols Used in This Manual and Their MeaningQualification of Personnel Designated UseGeneral Safety Instructions Safety and DesignUnpacking and Installation Opening the packaging Transport to the Installation Location Ramp assemblyRemoving the packaging Microtome knives Safety FeaturesSecuring/Locking the Handwheel Knife guardCleaning, Disinfection Turning the Instrument Back On Handling Specimens Defrosting MaintenanceRemoval of the Microtome Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 Hz Technical DataSet of filters 5 pieces Standard DeliveryHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Site Requirements InstallationRight side Left sideTransport to the Site Assembling the Handwheel Centering the Handwheel Optional Locking/Unlocking the HandwheelEmergency-stop function active, or Existing power system Connection to Power Supply SystemOnly for instruments sold in Japan Selecting the frequency Installation Shelf, Movable Optional Installing the Storage Systems Optional For reasonsInserting the Section Waste Tray Section waste tray with the a Cutout a facing the userInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Control Panel Fields on the CM1950 Control Panel Field Instrument Controls12 o’clock Position 6 o’clock 0μm Retraction20.0μm 60.0μmMotion continues as long as the button is held Coarse feed functionsBrief press of the button retracts the specimen by 20μm Sectioning modes Control panel 3 Motorized sectioning optionalLeica Cryofect is not available in all countries Disinfection Duration 30min Duration 180 minTurning the Instrument On Working with the InstrumentConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Shut off VAC LED in VAC button goes out Cutting with Extraction Anti-roll Guide installed10μm 40% 60% 80% 100% 20μm 30μm 40μm SpeedSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Working with the Instrument Problems During Work TroubleshootingProblem Causes Remedies Cryochamber Sections tear Alternately Longer sufficient Tissue type Chamber temperature Temperature Selection Chart in minus COrdering Information Optional AccessoriesCryofect, VPE Leica Cryofect 4x 350ml With anti-roll guide When using low-profile blades, the rulerLever 6 for the lateral shift Lever 7 for clamping the bladeBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Unscrew the bolts 35 of the pressure plate Cleaning the knife holder CE Daily cleaningNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories General Maintenance Maintenance and CleaningChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp Decontamination Certificate master This equipment has been cleaned and decontaminatedNB Microtome knives must be in boxes Yes No indicated belowBU Return Authorization Number EC Declaration of Conformity Anne De Greef-Safft President Biosystems DivisionWarranty and Service Peoples Republic of China