Leica CM1950 manual 12 o’clock Position 6 o’clock

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7. Instrument Controls

A

B12 o’clock

Position 6 o’clock

Press the "VAC" button to enable the vacuum extractor. The LED in the "VAC" button is lit while the extractor is on. Press the button again to disable it.

Use the knob to adjust the intensity of the vacuum.

A

Optimal area for trimming and sectioning

Trimming: Handwheel position 12 - 6 o’clock, valve open

 

Handwheel position

6 - 12 o’clock, valve closed

Cutting: Handwheel position 12 - 3 o’clock, valve open all the way

 

Handwheel position

3 - 6 o’clock, valve half open

 

Handwheel position

6 - 12 o’clock, valve closed

B

Optimal area for extraction from the chamber

To clean the chamber, turn the knob to the red range.

The strength of the required extraction force depends on the following:

Size of the specimen

Sectioning speed

Section thickness used

The Peltier element provides additional cooling for the freezing stations.

After the button is pressed, the display changes from "PE" to 10", indicating an additional cooling period of 10 minutes. The remaining cool- ing time is always shown in the display. Once only 4 minutes are remain-

ing, a dot will appear after the "4". As of this time, the Peltier element may be switched off early by pressing the button again.

Caution:

The specimen head and Peltier do not switch on until the chamber temperature reaches -5°C, in order to prevent icing.

If the condenser (resting phase) is off and the Peltier is activated, the digit 10 flashes until the condenser switches on again to pre- vent the Peltier from being destroyed when the condenser is not running. When the condenser starts up, the flashing starts and the 10 minutes are counted down.

(For exact instructions for using the chamber, specimen head and real time display fields, refer to the chapter on "Working with the Instrument" on page 37 of this Operating Manual.)

Leica CM1950 Cryostat

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Contents Leica CM1950 Page Page Table of contents 12.3 11.112.1 12.2Designated Use Symbols Used in This Manual and Their MeaningImportant Information Qualification of PersonnelGeneral Safety Instructions Safety and DesignUnpacking and Installation Opening the packaging Transport to the Installation Location Ramp assemblyRemoving the packaging Microtome knives Safety FeaturesSecuring/Locking the Handwheel Knife guardCleaning, Disinfection Turning the Instrument Back On Handling Specimens Defrosting MaintenanceRemoval of the Microtome Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 Hz Technical DataSet of filters 5 pieces Standard DeliveryHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Left side InstallationSite Requirements Right sideTransport to the Site Assembling the Handwheel Centering the Handwheel Optional Locking/Unlocking the HandwheelEmergency-stop function active, or Existing power system Connection to Power Supply SystemOnly for instruments sold in Japan Selecting the frequency Installation Section waste tray with the a Cutout a facing the user Installing the Storage Systems Optional For reasonsShelf, Movable Optional Inserting the Section Waste TrayInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Control Panel Fields on the CM1950 Control Panel Field Instrument Controls12 o’clock Position 6 o’clock 60.0μm Retraction0μm 20.0μmMotion continues as long as the button is held Coarse feed functionsBrief press of the button retracts the specimen by 20μm Sectioning modes Control panel 3 Motorized sectioning optionalLeica Cryofect is not available in all countries Disinfection Duration 30min Duration 180 minTurning the Instrument On Working with the InstrumentConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Shut off VAC LED in VAC button goes out Cutting with Extraction Anti-roll Guide installed10μm 40% 60% 80% 100% 20μm 30μm 40μm SpeedSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Working with the Instrument Problems During Work TroubleshootingProblem Causes Remedies Cryochamber Sections tear Alternately Longer sufficient Tissue type Chamber temperature Temperature Selection Chart in minus COrdering Information Optional AccessoriesCryofect, VPE Leica Cryofect 4x 350ml Lever 7 for clamping the blade When using low-profile blades, the rulerWith anti-roll guide Lever 6 for the lateral shiftBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Unscrew the bolts 35 of the pressure plate Cleaning the knife holder CE Daily cleaningNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories General Maintenance Maintenance and CleaningChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp Yes No indicated below This equipment has been cleaned and decontaminatedDecontamination Certificate master NB Microtome knives must be in boxesBU Return Authorization Number EC Declaration of Conformity Anne De Greef-Safft President Biosystems DivisionWarranty and Service Peoples Republic of China