Leica Official Manual for Operating the CM1950 Projection Television

Page 38

8.Working with the Instrument

Programming the specimen temperature

Select the desired temperature of the specimen.

The specimen temperature is set and indicated on the control panel marked with the specimen head symbol.

The actual temperature is the standard indication.

Briefly pressing the "+" or "-" button displays the target temperature.

Set the desired value via the "+" / "-" buttons. Pushing the "+" or "-" button for more than 1 sec. increases or decreases the specimen temperature continuously.

The actual value will be indicated 5 seconds after finishing the program- ming.

Caution:

The specimen head and Peltier do not switch on until the chamber temperature reaches -5°C, in order to prevent icing.

Specimen temperature - "Max-Cool" function

The snowflake button for the Max-Cool function is in the panel with the

specimen head symbol.

Pressing the button sets the lowest possible specimen head tem- perature (-50°C) as the target temperature. The instrument adjusts the maximum low temperature of the specimen head, i.e. -50°C.

Push the snowflake button again to stop the "Max-Cool" function.

The temperature adjusts to the value programmed prior to activating the "Max-Cool"-function.

Alternate flashing of "LL" and the actual temperature indicates activa- tion of the Max-Cool function.

Setting the time

The clock time is set with the +/- buttons in the control panel marked with the clock symbol.

To do so, set the current time using the "+" or "-" button below the small clock symbol.

Pushing the "+" or "-" button for more than 1 sec. increases or decreases the time continuously (auto-repeat function).

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Operating Manual V1.1 – 05/2007

Image 38
Contents Leica CM1950 Page Page Table of contents 12.2 11.112.1 12.3Qualification of Personnel Symbols Used in This Manual and Their MeaningImportant Information Designated UseUnpacking and Installation Safety and DesignGeneral Safety Instructions Opening the packaging Removing the packaging Ramp assemblyTransport to the Installation Location Safety Features Microtome knivesKnife guard Securing/Locking the HandwheelCleaning, Disinfection Turning the Instrument Back On Removal of the Microtome MaintenanceHandling Specimens Defrosting Technical Data Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 HzStandard Delivery Set of filters 5 piecesHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Right side InstallationSite Requirements Left sideTransport to the Site Assembling the Handwheel Emergency-stop function active, or Locking/Unlocking the HandwheelCentering the Handwheel Optional Only for instruments sold in Japan Selecting the frequency Connection to Power Supply SystemExisting power system Installation Inserting the Section Waste Tray Installing the Storage Systems Optional For reasonsShelf, Movable Optional Section waste tray with the a Cutout a facing the userInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Instrument Controls Control Panel Fields on the CM1950 Control Panel Field12 o’clock Position 6 o’clock 20.0μm Retraction0μm 60.0μmBrief press of the button retracts the specimen by 20μm Coarse feed functionsMotion continues as long as the button is held Control panel 3 Motorized sectioning optional Sectioning modesDisinfection Duration 30min Duration 180 min Leica Cryofect is not available in all countriesWorking with the Instrument Turning the Instrument OnConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Cutting with Extraction Anti-roll Guide installed Shut off VAC LED in VAC button goes outSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Speed10μm 40% 60% 80% 100% 20μm 30μm 40μm Working with the Instrument Problem Causes Remedies TroubleshootingProblems During Work Cryochamber Sections tear Alternately Longer sufficient Temperature Selection Chart in minus C Tissue type Chamber temperatureOptional Accessories Ordering InformationCryofect, VPE Leica Cryofect 4x 350ml Lever 6 for the lateral shift When using low-profile blades, the rulerWith anti-roll guide Lever 7 for clamping the bladeBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Cleaning the knife holder CE Daily cleaning Unscrew the bolts 35 of the pressure plateNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories Maintenance and Cleaning General MaintenanceChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp NB Microtome knives must be in boxes This equipment has been cleaned and decontaminatedDecontamination Certificate master Yes No indicated belowBU Return Authorization Number Anne De Greef-Safft President Biosystems Division EC Declaration of ConformityWarranty and Service Peoples Republic of China