Leica CM1950 Important Information, Symbols Used in This Manual and Their Meaning, Designated Use

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1.Important Information

1.1Symbols Used in This Manual and Their Meaning

Warnings

appear in a gray box and are marked by a warning triangle.

Notes

i.e. important user information appear in a gray box and are marked by an in-

formation symbol.

Caution – UVC radiation!

(5)Figures in brackets refer to item num-

(Fig.5)

bers in drawings or to the drawings

 

themselves.

1.2Qualification of Personnel

The Leica CM1950 may be operated by trained laboratory personnel only.

All laboratory personnel designated to operate the instrument must carefully read the present instruction manual prior to starting work with the instrument.

Despite chemical and/or UV-light dis- infection, personal safety precautions as per the applicable laboratory regu- lations must still be taken (i.e. safety goggles, gloves, laboratory coat and mask must be worn).

This type of disinfection reduces the number of germs by at least 99.99%.

1.3Designated Use

The CM1950 is a high-performance cryostat with an encapsulated microtome and separate speci- men cooling. It features a UV disinfection system, an (optional) integrated extraction system for sec- tion waste and an (optional) motor for motorized sectioning.

Thecryostatisdesignedtoproducefrozensections for biological, medical and industrial applications.

The CM1950 is suitable for in-vitro diagnostic (IVD) applications.

The instrument may only be operated within the scope of its designated use as described above and as per the instructions given in this manual.

Any other use of this instrument is considered

as improper operation.

1.4Instrument Type

All information given in this instruction manual applies only to the instrument type indicated on the title page.

A name plate, indicating the instrument serial number, is attached to the back of the instrument.

Fig. 1

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Operating Manual V1.1 – 05/2007

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Contents Leica CM1950 Page Page Table of contents 12.2 11.112.1 12.3Qualification of Personnel Symbols Used in This Manual and Their MeaningImportant Information Designated UseSafety and Design General Safety InstructionsUnpacking and Installation Opening the packaging Ramp assembly Transport to the Installation LocationRemoving the packaging Safety Features Microtome knivesKnife guard Securing/Locking the HandwheelCleaning, Disinfection Turning the Instrument Back On Maintenance Handling Specimens DefrostingRemoval of the Microtome Technical Data Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 HzStandard Delivery Set of filters 5 piecesHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Right side InstallationSite Requirements Left sideTransport to the Site Assembling the Handwheel Locking/Unlocking the Handwheel Centering the Handwheel OptionalEmergency-stop function active, or Connection to Power Supply System Existing power systemOnly for instruments sold in Japan Selecting the frequency Installation Inserting the Section Waste Tray Installing the Storage Systems Optional For reasonsShelf, Movable Optional Section waste tray with the a Cutout a facing the userInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Instrument Controls Control Panel Fields on the CM1950 Control Panel Field12 o’clock Position 6 o’clock 20.0μm Retraction0μm 60.0μmCoarse feed functions Motion continues as long as the button is heldBrief press of the button retracts the specimen by 20μm Control panel 3 Motorized sectioning optional Sectioning modesDisinfection Duration 30min Duration 180 min Leica Cryofect is not available in all countriesWorking with the Instrument Turning the Instrument OnConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Cutting with Extraction Anti-roll Guide installed Shut off VAC LED in VAC button goes outSpeed 10μm 40% 60% 80% 100% 20μm 30μm 40μmSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Working with the Instrument Troubleshooting Problems During WorkProblem Causes Remedies Cryochamber Sections tear Alternately Longer sufficient Temperature Selection Chart in minus C Tissue type Chamber temperatureOptional Accessories Ordering InformationCryofect, VPE Leica Cryofect 4x 350ml Lever 6 for the lateral shift When using low-profile blades, the rulerWith anti-roll guide Lever 7 for clamping the bladeBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Cleaning the knife holder CE Daily cleaning Unscrew the bolts 35 of the pressure plateNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories Maintenance and Cleaning General MaintenanceChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp NB Microtome knives must be in boxes This equipment has been cleaned and decontaminatedDecontamination Certificate master Yes No indicated belowBU Return Authorization Number Anne De Greef-Safft President Biosystems Division EC Declaration of ConformityWarranty and Service Peoples Republic of China