Leica CM1950 manual Locking/Unlocking the Handwheel, Centering the Handwheel Optional

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6. Installation

6.3.1 Locking/Unlocking the Handwheel

12 o’clock position

6 o’clock position

Fig. 15

1

2

Fig. 16

Only rotate the handwheel when the refrigeration system is on and the cryochamber is cold!

Always lock the handwheel prior to manipulating the knife or specimen, changing the specimen, or taking a break.

To lock the handwheel, move its handle to the 12 or 6 o’clock position. Press the lever (1) fully outward; gently rock the handwheel until the locking mech- anism clicks into place noticeably.

To release the handwheel, press the lever (2) on the handwheel toward the cryostat housing.

Centering the Handwheel (Optional)

An important safety device on the cryostat is the feature for center- ing the handwheel in motorized sectioning mode.

To do so, pull out the handwheel’s handle and position it in the middle of the handwheel. The handle automatically engages in this position.

Fig. 17

6.3.2 Installing the Footswitch Dummy (Instruments with Cutting Motor)

The footswitch dummy must be installed on the outer right side of the instrument (see page 16, General overview) if no footswitch (optional) is used.

If the red LED in the E-STOP field in control panel field 3 is illuminated, either:

emergency-stop function active, or

footswitch dummy (optional footswitch) connected incorrectly or not at all.

Fig. 18

Leica CM1950 Cryostat

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Image 23
Contents Leica CM1950 Page Page Table of contents 12.3 11.112.1 12.2Designated Use Symbols Used in This Manual and Their MeaningImportant Information Qualification of PersonnelUnpacking and Installation Safety and DesignGeneral Safety Instructions Opening the packaging Removing the packaging Ramp assemblyTransport to the Installation Location Microtome knives Safety FeaturesSecuring/Locking the Handwheel Knife guardCleaning, Disinfection Turning the Instrument Back On Removal of the Microtome MaintenanceHandling Specimens Defrosting Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 Hz Technical DataSet of filters 5 pieces Standard DeliveryHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Left side InstallationSite Requirements Right sideTransport to the Site Assembling the Handwheel Emergency-stop function active, or Locking/Unlocking the HandwheelCentering the Handwheel Optional Only for instruments sold in Japan Selecting the frequency Connection to Power Supply SystemExisting power system Installation Section waste tray with the a Cutout a facing the user Installing the Storage Systems Optional For reasonsShelf, Movable Optional Inserting the Section Waste TrayInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Control Panel Fields on the CM1950 Control Panel Field Instrument Controls12 o’clock Position 6 o’clock 60.0μm Retraction0μm 20.0μmBrief press of the button retracts the specimen by 20μm Coarse feed functionsMotion continues as long as the button is held Sectioning modes Control panel 3 Motorized sectioning optionalLeica Cryofect is not available in all countries Disinfection Duration 30min Duration 180 minTurning the Instrument On Working with the InstrumentConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Shut off VAC LED in VAC button goes out Cutting with Extraction Anti-roll Guide installedSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Speed10μm 40% 60% 80% 100% 20μm 30μm 40μm Working with the Instrument Problem Causes Remedies TroubleshootingProblems During Work Cryochamber Sections tear Alternately Longer sufficient Tissue type Chamber temperature Temperature Selection Chart in minus COrdering Information Optional AccessoriesCryofect, VPE Leica Cryofect 4x 350ml Lever 7 for clamping the blade When using low-profile blades, the rulerWith anti-roll guide Lever 6 for the lateral shiftBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Unscrew the bolts 35 of the pressure plate Cleaning the knife holder CE Daily cleaningNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories General Maintenance Maintenance and CleaningChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp Yes No indicated below This equipment has been cleaned and decontaminatedDecontamination Certificate master NB Microtome knives must be in boxesBU Return Authorization Number EC Declaration of Conformity Anne De Greef-Safft President Biosystems DivisionWarranty and Service Peoples Republic of China