Leica CM1950 manual

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1. NOTE

The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state- of-the-art technology as we understand it follow- ing thorough investigation in this field. We are under no obligation to update the present manu- al periodically and on an ongoing basis accord- ing to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.

For erroneous statements, drawings, technical illustrations etc. contained in this manual we ex- clude liability as far as permissible according to the national legal system applicable in each indi- vidual case. In particular, no liability whatsoever is accepted for any financial loss or consequen- tial damage caused by or related to compliance with statements or other information in this manual.

Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products.

These are determined only by the contract provi- sions agreed between ourselves and our customers.

Leica reserves the right to change technical spec- ifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and man- ufacturing techniques used in our products.

This document is protected under copyright laws. All copyrights to this documentation are held by Leica Biosystems Nussloch GmbH.

Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods – includ- ing any electronic systems and media – requires express prior permission in writing by Leica Biosystems Nussloch GmbH.

For the instrument serial number and year of man- ufacture, please refer to the name plate at the back of the instrument.

© Leica Biosystems Nussloch GmbH

Published by:

Leica Biosystems Nussloch GmbH

Heidelberger Str. 17 - 19

D-69226 Nussloch

Germany

Phone: +49 (6224) 143-0

Fax: +49 (6224) 143-200

eMail: histo_info@leica-microsystems.com

Internet: http://www.histo-solutions.com

Leica CM1950 – Cryostat

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Contents Leica CM1950 Page Page Table of contents 12.3 11.112.1 12.2Designated Use Symbols Used in This Manual and Their MeaningImportant Information Qualification of PersonnelSafety and Design General Safety InstructionsUnpacking and Installation Opening the packaging Ramp assembly Transport to the Installation LocationRemoving the packaging Microtome knives Safety FeaturesSecuring/Locking the Handwheel Knife guardCleaning, Disinfection Turning the Instrument Back On Maintenance Handling Specimens DefrostingRemoval of the Microtome Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 Hz Technical DataSet of filters 5 pieces Standard DeliveryHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Left side InstallationSite Requirements Right sideTransport to the Site Assembling the Handwheel Locking/Unlocking the Handwheel Centering the Handwheel OptionalEmergency-stop function active, or Connection to Power Supply System Existing power systemOnly for instruments sold in Japan Selecting the frequency Installation Section waste tray with the a Cutout a facing the user Installing the Storage Systems Optional For reasonsShelf, Movable Optional Inserting the Section Waste TrayInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Control Panel Fields on the CM1950 Control Panel Field Instrument Controls12 o’clock Position 6 o’clock 60.0μm Retraction0μm 20.0μmCoarse feed functions Motion continues as long as the button is heldBrief press of the button retracts the specimen by 20μm Sectioning modes Control panel 3 Motorized sectioning optionalLeica Cryofect is not available in all countries Disinfection Duration 30min Duration 180 minTurning the Instrument On Working with the InstrumentConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Shut off VAC LED in VAC button goes out Cutting with Extraction Anti-roll Guide installedSpeed 10μm 40% 60% 80% 100% 20μm 30μm 40μmSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Working with the Instrument Troubleshooting Problems During WorkProblem Causes Remedies Cryochamber Sections tear Alternately Longer sufficient Tissue type Chamber temperature Temperature Selection Chart in minus COrdering Information Optional AccessoriesCryofect, VPE Leica Cryofect 4x 350ml Lever 7 for clamping the blade When using low-profile blades, the rulerWith anti-roll guide Lever 6 for the lateral shiftBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Unscrew the bolts 35 of the pressure plate Cleaning the knife holder CE Daily cleaningNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories General Maintenance Maintenance and CleaningChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp Yes No indicated below This equipment has been cleaned and decontaminatedDecontamination Certificate master NB Microtome knives must be in boxesBU Return Authorization Number EC Declaration of Conformity Anne De Greef-Safft President Biosystems DivisionWarranty and Service Peoples Republic of China