Leica manual Technical Data, Refrigeration system CM1950, 50 Hz CM1950, 60 Hz

Page 15

 

 

3.

Technical Data

 

 

 

Refrigeration system

CM1950, 50 Hz

CM1950, 60 Hz

Cryochamber

 

 

 

Temperature range

0°C to -35°C ± 5 K, adjustable in 1K increments,

0°C to -35°C ± 5 K, adjustable in 1K increments

 

at ambient temperature of 20°C

ambient temperature of 20°C

Cooling time to -25°C

approx. 5 h

approx. 5 h

 

Cooling time to -35°C

approx. 8 h

approx. 8 h

 

Refrigeration capacity

690 W

690 W

 

Cut-out pressure

25 bar

25 bar

 

Safety factor

3

3

 

Refrigerant*

300 g (± 5 g) refrigerant R -404A*

300 g (± 5 g) refrigerant R -404A*

Compressor oil*

0.6 l EMKARATE RL22S, ICI*

0.6 l EMKARATE RL22S, ICI*

Defrosting of cryochamber

 

 

 

Automatic defrosting

 

 

 

Programmable:

Yes (hot gas defrost) Selectable time

Yes (hot gas defrost) Selectable time

Defrosting intervals

1 defrost in 24 h

1 defrost in 24 h

 

 

or manual hot gas defrost

or manual hot gas defrost

Defrost time:

12 minutes

12 minutes

 

Automatic shutoff Defrost:

at -5 °C chamber temperature

at -5 °C chamber temperature

Quick-freeze shelf

 

 

 

Minimum temperature:

- 42 °C (+ 5 K), at chamber temp. -35°C

- 42 °C (+ 5 K), at chamber temp. -35°C

Number of freezing stations:

15+2

15+2

 

Defrost

Manual hot-gas defrost

Manual hot-gas defrost

Peltier element

 

 

 

Number of freezing stations:

2

2

 

Max. temperature difference:

17 K, at chamber temp. of -35°C

17 K, at chamber temp. of -35°C

Specimen cooling

 

 

 

Temperature range

-10 to -50 °C ± 3 K

-10 to -50 °C ± 3 K

 

Refrigeration capacity

320 W

320 W

 

Cut-out pressure

25 bar

25 bar

 

Safety factor

3

3

 

Refrigerant and quantity

at 230V/50 Hz 140 g (± 5 g), refrigerant R-404A*

at 120V/60 Hz 110 g (± 5 g), refrigerant R-404A*

 

at 100V/50/60 Hz 110 g (± 5 g), refrigerant R-404A*

 

 

Compressor oil*

0.4 l alpha 22, Kyodo*

0.4 l alpha 22, Kyodo*

Defrosting of specimen head

 

 

 

Automatic defrost:

No

No

 

Manual defrost

 

 

 

Defrost time

15 min.

15 min.

 

*) Refrigerant and compressor oil must be replaced by qualified, authorized service personnel only.

acc. to CECOMAF: liquid temperature 45 °C, evaporation temperature: -25 °C

Leica CM1950 – Cryostat

15

Image 15
Contents Leica CM1950 Page Page Table of contents 12.3 11.112.1 12.2Designated Use Symbols Used in This Manual and Their MeaningImportant Information Qualification of PersonnelSafety and Design General Safety InstructionsUnpacking and Installation Opening the packaging Ramp assembly Transport to the Installation LocationRemoving the packaging Microtome knives Safety FeaturesSecuring/Locking the Handwheel Knife guardCleaning, Disinfection Turning the Instrument Back On Maintenance Handling Specimens DefrostingRemoval of the Microtome Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 Hz Technical DataSet of filters 5 pieces Standard DeliveryHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Left side InstallationSite Requirements Right sideTransport to the Site Assembling the Handwheel Locking/Unlocking the Handwheel Centering the Handwheel OptionalEmergency-stop function active, or Connection to Power Supply System Existing power systemOnly for instruments sold in Japan Selecting the frequency Installation Section waste tray with the a Cutout a facing the user Installing the Storage Systems Optional For reasonsShelf, Movable Optional Inserting the Section Waste TrayInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Control Panel Fields on the CM1950 Control Panel Field Instrument Controls12 o’clock Position 6 o’clock 60.0μm Retraction0μm 20.0μmCoarse feed functions Motion continues as long as the button is heldBrief press of the button retracts the specimen by 20μm Sectioning modes Control panel 3 Motorized sectioning optionalLeica Cryofect is not available in all countries Disinfection Duration 30min Duration 180 minTurning the Instrument On Working with the InstrumentConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Shut off VAC LED in VAC button goes out Cutting with Extraction Anti-roll Guide installedSpeed 10μm 40% 60% 80% 100% 20μm 30μm 40μmSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Working with the Instrument Troubleshooting Problems During WorkProblem Causes Remedies Cryochamber Sections tear Alternately Longer sufficient Tissue type Chamber temperature Temperature Selection Chart in minus COrdering Information Optional AccessoriesCryofect, VPE Leica Cryofect 4x 350ml Lever 7 for clamping the blade When using low-profile blades, the rulerWith anti-roll guide Lever 6 for the lateral shiftBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Unscrew the bolts 35 of the pressure plate Cleaning the knife holder CE Daily cleaningNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories General Maintenance Maintenance and CleaningChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp Yes No indicated below This equipment has been cleaned and decontaminatedDecontamination Certificate master NB Microtome knives must be in boxesBU Return Authorization Number EC Declaration of Conformity Anne De Greef-Safft President Biosystems DivisionWarranty and Service Peoples Republic of China