Leica CM1950 manual Will result

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11. Optional Accessories

Adjusting the Knife Holder with Anti-roll Guide You can adjust the height of the anti-roll guide system using the knurled nut (1):

If you turn the nut counterclockwise, the anti- roll guide system moves toward the blade.

If you turn the nut clockwise, the anti-roll guide system moves away from the blade.

1

If the anti-roll guide system is in the wrong posi- tion relative to the cutter, the following problems

will result:

Blade holder CE with glass anti-roll guide

Fig. 47

Fig. I: The section rolls over the glass insert of the anti-roll guide system.

Error: Glass insert not high enough.

Remedy: Turn the knurled nut counterclockwise until the section is pushed between the blade and anti-roll guide as shown in Fig. III.

I

Fig. II: Section tears and block hits the glass insert after sectioning. The section rolls over the glass insert of the anti-roll guide system.

Error: Anti-roll guide system is set too high.

Remedy: Turn the knurled nut clockwise until the section is pushed between the blade

IIand anti-roll guide as shown in Fig. III.

Generally, we recommend pre-adjusting the anti-roll guide system at a high sec- tion thickness (e.g. 10μm). Start from there and work your way down to the desired section thickness in small increments, readjusting the anti-roll guide system at each increment using the knurled nut.

III

Fig. 48,

Knife holder CN with glass anti-roll guide

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Operating Manual V1.1 – 05/2007

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Contents Leica CM1950 Page Page Table of contents 12.2 11.112.1 12.3Qualification of Personnel Symbols Used in This Manual and Their MeaningImportant Information Designated UseGeneral Safety Instructions Safety and DesignUnpacking and Installation Opening the packaging Transport to the Installation Location Ramp assemblyRemoving the packaging Safety Features Microtome knivesKnife guard Securing/Locking the HandwheelCleaning, Disinfection Turning the Instrument Back On Handling Specimens Defrosting MaintenanceRemoval of the Microtome Technical Data Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 HzStandard Delivery Set of filters 5 piecesHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Right side InstallationSite Requirements Left sideTransport to the Site Assembling the Handwheel Centering the Handwheel Optional Locking/Unlocking the HandwheelEmergency-stop function active, or Existing power system Connection to Power Supply SystemOnly for instruments sold in Japan Selecting the frequency Installation Inserting the Section Waste Tray Installing the Storage Systems Optional For reasonsShelf, Movable Optional Section waste tray with the a Cutout a facing the userInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Instrument Controls Control Panel Fields on the CM1950 Control Panel Field12 o’clock Position 6 o’clock 20.0μm Retraction0μm 60.0μmMotion continues as long as the button is held Coarse feed functionsBrief press of the button retracts the specimen by 20μm Control panel 3 Motorized sectioning optional Sectioning modesDisinfection Duration 30min Duration 180 min Leica Cryofect is not available in all countriesWorking with the Instrument Turning the Instrument OnConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Cutting with Extraction Anti-roll Guide installed Shut off VAC LED in VAC button goes out10μm 40% 60% 80% 100% 20μm 30μm 40μm SpeedSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Working with the Instrument Problems During Work TroubleshootingProblem Causes Remedies Cryochamber Sections tear Alternately Longer sufficient Temperature Selection Chart in minus C Tissue type Chamber temperatureOptional Accessories Ordering InformationCryofect, VPE Leica Cryofect 4x 350ml Lever 6 for the lateral shift When using low-profile blades, the rulerWith anti-roll guide Lever 7 for clamping the bladeBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Cleaning the knife holder CE Daily cleaning Unscrew the bolts 35 of the pressure plateNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories Maintenance and Cleaning General MaintenanceChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp NB Microtome knives must be in boxes This equipment has been cleaned and decontaminatedDecontamination Certificate master Yes No indicated belowBU Return Authorization Number Anne De Greef-Safft President Biosystems Division EC Declaration of ConformityWarranty and Service Peoples Republic of China