Leica CM1950 manual Handwheel, motorized

Page 17

4. Standard Delivery

Basic instrument WITH motor/WITHOUT extraction, in the specific voltage variant

1 handwheel, motorized	0477 41347
5 specimen discs, 30mm	0477 40044
1 section waste tray	0477 40062
1 position holder for freeze shelf	0477 40080
1 freeze shelf cover	0477 43763
1 tool set	0436 43463
- 1 brush, fine	0183 28642
- 1 Leica brush w/magnet	0183 40426
- 1 Allen key, No. 1.5	0222 10050
- 1 Allen key, No. 2.5	0222 04137
- 1 Allen key, No. 3.0	0222 04138
- 1 Allen key, No. 4.0	0222 04139
- 1 Allen key with dome head, No. 4.0	0222 32131
- 1 Allen key, No. 5.0	0222 04140
- 1 key with handle, No. 5.0	0194 04760
- 1 Allen key, No. 6.0	0222 04141
- 1 Single-head wrench, No. 13	0330 33149
- 1 Single-head wrench, No. 16	0330 18595
1 power cable
1 bottle of cryostat oil, type 407, 50 ml	0336 06098
1 footswitch dummy	0443 30420
1 bottle of OCT freezing compound, 125 ml	0201 08926
1 pair of safety gloves, size M, * for cryosectioning	0340 29011
1 operating instructions plus language CD	0708 37115

*Note: for the Japanese version: 100V, 50/60 Hz; 1 pair of safety gloves, size S (0340 40859) is included.

Basic instrument WITH and WITH extraction, in the specific voltage variant
Standard scope of delivery as above,
plus:
1 accessory set (extraction)	0477 43300
- Hose adapter 1	0477 40293
- Hose adapter 2	0477 40294
- Suction nozzle	0477 40295
- Silicone hose	0477 43302
- Silicone stopper	0477 43304
- Chamber suction nozzle	0477 43779
- Set of filters (5 pieces)	0477 43792

Compare the delivered components with the parts list and your order. Should you find any discrepancies, please contact your Leica sales office without delay.

A choice of different knife holders is available for the CM1950.

Leica CM1950 – Cryostat

17

Image 17

Contents Leica CM1950 Page Page Table of contents 12.1 11.112.2 12.3Important Information Symbols Used in This Manual and Their MeaningQualification of Personnel Designated UseUnpacking and Installation Safety and DesignGeneral Safety Instructions Opening the packaging Removing the packaging Ramp assemblyTransport to the Installation Location Microtome knives Safety FeaturesSecuring/Locking the Handwheel Knife guardCleaning, Disinfection Turning the Instrument Back On Removal of the Microtome MaintenanceHandling Specimens Defrosting Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 Hz Technical DataSet of filters 5 pieces Standard DeliveryHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Site Requirements InstallationRight side Left sideTransport to the Site Assembling the Handwheel Emergency-stop function active, or Locking/Unlocking the HandwheelCentering the Handwheel Optional Only for instruments sold in Japan Selecting the frequency Connection to Power Supply SystemExisting power system Installation Shelf, Movable Optional Installing the Storage Systems Optional For reasonsInserting the Section Waste Tray Section waste tray with the a Cutout a facing the userInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Control Panel Fields on the CM1950 Control Panel Field Instrument Controls12 o’clock Position 6 o’clock 0μm Retraction20.0μm 60.0μmBrief press of the button retracts the specimen by 20μm Coarse feed functionsMotion continues as long as the button is held Sectioning modes Control panel 3 Motorized sectioning optionalLeica Cryofect is not available in all countries Disinfection Duration 30min Duration 180 minTurning the Instrument On Working with the InstrumentConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Shut off VAC LED in VAC button goes out Cutting with Extraction Anti-roll Guide installedSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Speed10μm 40% 60% 80% 100% 20μm 30μm 40μm Working with the Instrument Problem Causes Remedies TroubleshootingProblems During Work Cryochamber Sections tear Alternately Longer sufficient Tissue type Chamber temperature Temperature Selection Chart in minus COrdering Information Optional AccessoriesCryofect, VPE Leica Cryofect 4x 350ml With anti-roll guide When using low-profile blades, the rulerLever 6 for the lateral shift Lever 7 for clamping the bladeBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Unscrew the bolts 35 of the pressure plate Cleaning the knife holder CE Daily cleaningNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories General Maintenance Maintenance and CleaningChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp Decontamination Certificate master This equipment has been cleaned and decontaminatedNB Microtome knives must be in boxes Yes No indicated belowBU Return Authorization Number EC Declaration of Conformity Anne De Greef-Safft President Biosystems DivisionWarranty and Service Peoples Republic of China