Leica CM1950 manual Handwheel position 12 3 o’clock, valve open all the way

Page 44

8.Working with the Instrument

For motorized sectioning, always begin at low speed for safety reasons.

A

B

Optimize the vacuum settings if necessary.

Press the "VAC" button to enable the vacuum extractor. The LED in the "VAC" button is lit while the extractor is on. Press the button again to disable it.

Use the knob to adjust the intensity of the vacuum.

A

Optimal area for trimming and sectioning

Trimming:

Handwheel position 12 - 6 o’clock, valve open

 

 

Handwheel position

6 - 12 o’clock, valve closed

Cutting:

Handwheel position 12 - 3 o’clock, valve open all the way

 

 

Handwheel position

3 - 6 o’clock, valve half open

 

 

Handwheel position

6 - 12 o’clock, valve closed

B

Optimal area for extraction from the chamber

To clean the chamber, turn the knob to the red range.

If the handwheel is not moved for approx. 5 sec, the valves close and the fan remains on.

If the handwheel is moved for approx. 1 min, the valves close, the fan shuts off (the LED in the VAC switch goes off to prevent icing).

To continue working, you now have to reactivate the VAC.

E

2. Trimming with Extraction – Brush Technique, finger rest installed

Remove the silicone stopper (E) from the filter cover (and keep it in a safe place).

Insert the extraction hose with the black adapter.

Fasten the suction nozzle to the pressure plate (using 4 magnets on the rear side of the nozzle) as far as possible towards the blade.

Ensure that the hose with the nozzle is not installed against its "natural" curvature on the pressure plate of the blade holder.

The tension acting on the hose can be minimized by turning the red ring (Fig. 28 on page 28) clockwise so that the suction nozzle presses against the pressure plate.

Check that the suction nozzle is seated optimally (by gently turning the handwheel)

44

Operating Manual V1.1 – 05/2007

Image 44
Contents Leica CM1950 Page Page Table of contents 11.1 12.112.2 12.3Symbols Used in This Manual and Their Meaning Important InformationQualification of Personnel Designated UseUnpacking and Installation Safety and DesignGeneral Safety Instructions Opening the packaging Removing the packaging Ramp assemblyTransport to the Installation Location Safety Features Microtome knivesKnife guard Securing/Locking the HandwheelCleaning, Disinfection Turning the Instrument Back On Removal of the Microtome MaintenanceHandling Specimens Defrosting Technical Data Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 HzStandard Delivery Set of filters 5 piecesHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Installation Site RequirementsRight side Left sideTransport to the Site Assembling the Handwheel Emergency-stop function active, or Locking/Unlocking the HandwheelCentering the Handwheel Optional Only for instruments sold in Japan Selecting the frequency Connection to Power Supply SystemExisting power system Installation Installing the Storage Systems Optional For reasons Shelf, Movable OptionalInserting the Section Waste Tray Section waste tray with the a Cutout a facing the userInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Instrument Controls Control Panel Fields on the CM1950 Control Panel Field12 o’clock Position 6 o’clock Retraction 0μm20.0μm 60.0μmBrief press of the button retracts the specimen by 20μm Coarse feed functionsMotion continues as long as the button is held Control panel 3 Motorized sectioning optional Sectioning modesDisinfection Duration 30min Duration 180 min Leica Cryofect is not available in all countriesWorking with the Instrument Turning the Instrument OnConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Cutting with Extraction Anti-roll Guide installed Shut off VAC LED in VAC button goes outSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Speed10μm 40% 60% 80% 100% 20μm 30μm 40μm Working with the Instrument Problem Causes Remedies TroubleshootingProblems During Work Cryochamber Sections tear Alternately Longer sufficient Temperature Selection Chart in minus C Tissue type Chamber temperatureOptional Accessories Ordering InformationCryofect, VPE Leica Cryofect 4x 350ml When using low-profile blades, the ruler With anti-roll guideLever 6 for the lateral shift Lever 7 for clamping the bladeBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Cleaning the knife holder CE Daily cleaning Unscrew the bolts 35 of the pressure plateNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories Maintenance and Cleaning General MaintenanceChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp This equipment has been cleaned and decontaminated Decontamination Certificate masterNB Microtome knives must be in boxes Yes No indicated belowBU Return Authorization Number Anne De Greef-Safft President Biosystems Division EC Declaration of ConformityWarranty and Service Peoples Republic of China