Leica CM1950 manual Not Visible

Page 60

11. Optional Accessories

1

3a

 

3a

not

2

 

visible

 

 

3

4

4

5

6

Fig. 51

Knife holder CN with anti-roll guide – Moving the clamping chucks

The specimen disc 50x80mm is suitable only for section thicknesses up to approx. 5μm (due to the large specimen size).

The large specimen disc (80x50mm) should pref- erably be used with knife holder CN and the 16cm C-profile steel knife.

The clamping jaws are factory-installed in the knife holder with a clearance of 64mm. If neces- sary, both clamping chucks can be offset with a clearance of 84mm.

Perform the following steps:

Use a 3 mm Allen key to loosen the screw over the clearance angle adjustment (1) and remove the segment arch (2) from the knife holder base.

Use a 3 mm Allen key to loosen the screws (4) on the underside of the segment arch.

Carefully pull the clamping jaw (5) on the right upward and insert it in the neighboring hole (6). Tighten the screws from the underside of the segment arch. Repeat on the left side.

Insert the long knife support (3) on the side over the knurled screw (3a) so that the recess faces the user – turn the height adjustment knurled screws until the lower stop is reached.

The knife can now be inserted from the side and its height adjusted via the knurled screws (3a).

Never work with only one clamping jaw, as this does not ensure the stability required for the sectioning process. Also, a long knife will not be sufficiently protected by the knife guard in this instance.

60

Operating Manual V1.1 – 05/2007

Image 60
Contents Leica CM1950 Page Page Table of contents 11.1 12.112.2 12.3Symbols Used in This Manual and Their Meaning Important InformationQualification of Personnel Designated UseSafety and Design General Safety InstructionsUnpacking and Installation Opening the packaging Ramp assembly Transport to the Installation LocationRemoving the packaging Safety Features Microtome knivesKnife guard Securing/Locking the HandwheelCleaning, Disinfection Turning the Instrument Back On Maintenance Handling Specimens DefrostingRemoval of the Microtome Technical Data Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 HzStandard Delivery Set of filters 5 piecesHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Installation Site RequirementsRight side Left sideTransport to the Site Assembling the Handwheel Locking/Unlocking the Handwheel Centering the Handwheel OptionalEmergency-stop function active, or Connection to Power Supply System Existing power systemOnly for instruments sold in Japan Selecting the frequency Installation Installing the Storage Systems Optional For reasons Shelf, Movable OptionalInserting the Section Waste Tray Section waste tray with the a Cutout a facing the userInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Instrument Controls Control Panel Fields on the CM1950 Control Panel Field12 o’clock Position 6 o’clock Retraction 0μm20.0μm 60.0μmCoarse feed functions Motion continues as long as the button is heldBrief press of the button retracts the specimen by 20μm Control panel 3 Motorized sectioning optional Sectioning modesDisinfection Duration 30min Duration 180 min Leica Cryofect is not available in all countriesWorking with the Instrument Turning the Instrument OnConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Cutting with Extraction Anti-roll Guide installed Shut off VAC LED in VAC button goes outSpeed 10μm 40% 60% 80% 100% 20μm 30μm 40μmSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Working with the Instrument Troubleshooting Problems During WorkProblem Causes Remedies Cryochamber Sections tear Alternately Longer sufficient Temperature Selection Chart in minus C Tissue type Chamber temperatureOptional Accessories Ordering InformationCryofect, VPE Leica Cryofect 4x 350ml When using low-profile blades, the ruler With anti-roll guideLever 6 for the lateral shift Lever 7 for clamping the bladeBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Cleaning the knife holder CE Daily cleaning Unscrew the bolts 35 of the pressure plateNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories Maintenance and Cleaning General MaintenanceChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp This equipment has been cleaned and decontaminated Decontamination Certificate masterNB Microtome knives must be in boxes Yes No indicated belowBU Return Authorization Number Anne De Greef-Safft President Biosystems Division EC Declaration of ConformityWarranty and Service Peoples Republic of China