Leica CM1950 manual Maintenance and Cleaning, General Maintenance

Page 66

12.Maintenance and Cleaning

12.1 General Maintenance

The microtome is virtually maintenance-free. To ensure a smooth operation of the instrument over several years we recommend the following:

At least once a year, have the instrument inspected by a qualified service engineer authorized by Leica.

Enter into a service contract at the end of the warranty period. For further information, please contact your local Leica service center.

Clean the instrument daily.

Every day, remove frozen section waste from the cryostat using a cold brush or use the (optional) extraction device.

Remove the section waste tray for emptying.

Remove the storage shelves and the brush shelf for cleaning.

Remove the closed sliding window from the front by gently lifting it (see p. 63, "Replacing the Fluorescent Lamp").

Do not use organic solvents or any other aggressive substances for cleaning and disinfecting!

Use only disinfectants listed in this manual such as Leica Cryofect (alcohol or other common alcohol-based disinfectants).

Drain the cleaning liquid through the hose after the prescribed reagent time is over and collect it in the waste container (1).

Emptying the condensate bottle

Check the fill level of the condensate bottle (1) visible in the front panel of the instrument at regular intervals.

• The bottle collects the condensate that accumulates during defrosting.

Dispose of the contents of the bottle in accordance with laboratory regulations.

1

Fig. 67

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Operating Manual V1.1 – 05/2007

Image 66
Contents Leica CM1950 Page Page Table of contents 12.2 11.112.1 12.3Qualification of Personnel Symbols Used in This Manual and Their MeaningImportant Information Designated UseSafety and Design General Safety InstructionsUnpacking and Installation Opening the packaging Ramp assembly Transport to the Installation LocationRemoving the packaging Safety Features Microtome knivesKnife guard Securing/Locking the HandwheelCleaning, Disinfection Turning the Instrument Back On Maintenance Handling Specimens DefrostingRemoval of the Microtome Technical Data Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 HzStandard Delivery Set of filters 5 piecesHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Right side InstallationSite Requirements Left sideTransport to the Site Assembling the Handwheel Locking/Unlocking the Handwheel Centering the Handwheel OptionalEmergency-stop function active, or Connection to Power Supply System Existing power systemOnly for instruments sold in Japan Selecting the frequency Installation Inserting the Section Waste Tray Installing the Storage Systems Optional For reasonsShelf, Movable Optional Section waste tray with the a Cutout a facing the userInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Instrument Controls Control Panel Fields on the CM1950 Control Panel Field12 o’clock Position 6 o’clock 20.0μm Retraction0μm 60.0μmCoarse feed functions Motion continues as long as the button is heldBrief press of the button retracts the specimen by 20μm Control panel 3 Motorized sectioning optional Sectioning modesDisinfection Duration 30min Duration 180 min Leica Cryofect is not available in all countriesWorking with the Instrument Turning the Instrument OnConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Cutting with Extraction Anti-roll Guide installed Shut off VAC LED in VAC button goes outSpeed 10μm 40% 60% 80% 100% 20μm 30μm 40μmSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Working with the Instrument Troubleshooting Problems During WorkProblem Causes Remedies Cryochamber Sections tear Alternately Longer sufficient Temperature Selection Chart in minus C Tissue type Chamber temperatureOptional Accessories Ordering InformationCryofect, VPE Leica Cryofect 4x 350ml Lever 6 for the lateral shift When using low-profile blades, the rulerWith anti-roll guide Lever 7 for clamping the bladeBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Cleaning the knife holder CE Daily cleaning Unscrew the bolts 35 of the pressure plateNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories Maintenance and Cleaning General MaintenanceChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp NB Microtome knives must be in boxes This equipment has been cleaned and decontaminatedDecontamination Certificate master Yes No indicated belowBU Return Authorization Number Anne De Greef-Safft President Biosystems Division EC Declaration of ConformityWarranty and Service Peoples Republic of China