Leica CM1950 manual Mark for suction nozzle

Page 29

6. Installation

6.5.9 Installing the Section Extraction (Optional) – Use with Blade Holder CE Only

	A	D
	A	C
		C
B
	G
		F
		E
Fig. 29	H
Fig. 29

•Silicone hose (A) with hose adapter 1 (B, to the filter in the instrument), hose adapter 2 (C, for suction nozzle D or F) and suction nozzle (D) – factory pre- assembled

•Silicone stopper (E)

•Chamber suction nozzle (F)

•Filter (G)

•Plastic clip (H), for parking the chamber suction nozzle.

When the suction nozzles are changed, the adapt- er (white) remains in the silicone hose. Pull off the nozzle by rotating and pulling it gently and firmly plug in the desired nozzle.

Ensure that the hose with the nozzle is not installed against its "natural" cur- vature on the pressure plate of the blade holder.

The tension acting on the hose can be minimized by turning the red ring (Fig. 31, top right) clock- wise so that the suction nozzle presses against the pressure plate (I, Fig. 31).

Afterwards, fold the anti-roll guide (K) back onto the pressure plate.

Mark for suction nozzle

Fig. 30

	K	B
	K	D
		D
C		I
		A

Fig. 31

•The scope of delivery also includes 2 plastic clips (H). These enable comfortable "parking" of the chamber suction nozzle (F) during sec- tioning.

The clip must be glued in before switching on the refrigeration. Before doing so, briefly degrease the surface to ensure a secure hold.

Preferably, the clip should be attached outside the working area, e.g. on the left inside wall of the instrument.

If the extraction nozzle (D) is not being used, it can be "parked" on one of the two magnetic surfaces indicated in the interior of the instrument.

If the extraction is not used for a long time, it is absolutely necessary to clean the extraction hose in order to ensure maximum extraction capacity. To do so, place the hose in commercially available disinfectant or alcohol. After several cleanings, the hose must be replaced (see order information on p. 49)!

Leica CM1950 – Cryostat

29

Image 29

Contents Leica CM1950 Page Page Table of contents 12.1 11.112.2 12.3Important Information Symbols Used in This Manual and Their MeaningQualification of Personnel Designated UseUnpacking and Installation Safety and DesignGeneral Safety Instructions Opening the packaging Removing the packaging Ramp assemblyTransport to the Installation Location Microtome knives Safety FeaturesSecuring/Locking the Handwheel Knife guardCleaning, Disinfection Turning the Instrument Back On Removal of the Microtome MaintenanceHandling Specimens Defrosting Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 Hz Technical DataSet of filters 5 pieces Standard DeliveryHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Site Requirements InstallationRight side Left sideTransport to the Site Assembling the Handwheel Emergency-stop function active, or Locking/Unlocking the HandwheelCentering the Handwheel Optional Only for instruments sold in Japan Selecting the frequency Connection to Power Supply SystemExisting power system Installation Shelf, Movable Optional Installing the Storage Systems Optional For reasonsInserting the Section Waste Tray Section waste tray with the a Cutout a facing the userInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Control Panel Fields on the CM1950 Control Panel Field Instrument Controls12 o’clock Position 6 o’clock 0μm Retraction20.0μm 60.0μmBrief press of the button retracts the specimen by 20μm Coarse feed functionsMotion continues as long as the button is held Sectioning modes Control panel 3 Motorized sectioning optionalLeica Cryofect is not available in all countries Disinfection Duration 30min Duration 180 minTurning the Instrument On Working with the InstrumentConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Shut off VAC LED in VAC button goes out Cutting with Extraction Anti-roll Guide installedSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Speed10μm 40% 60% 80% 100% 20μm 30μm 40μm Working with the Instrument Problem Causes Remedies TroubleshootingProblems During Work Cryochamber Sections tear Alternately Longer sufficient Tissue type Chamber temperature Temperature Selection Chart in minus COrdering Information Optional AccessoriesCryofect, VPE Leica Cryofect 4x 350ml With anti-roll guide When using low-profile blades, the rulerLever 6 for the lateral shift Lever 7 for clamping the bladeBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Unscrew the bolts 35 of the pressure plate Cleaning the knife holder CE Daily cleaningNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories General Maintenance Maintenance and CleaningChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp Decontamination Certificate master This equipment has been cleaned and decontaminatedNB Microtome knives must be in boxes Yes No indicated belowBU Return Authorization Number EC Declaration of Conformity Anne De Greef-Safft President Biosystems DivisionWarranty and Service Peoples Republic of China