Leica CM1950 manual Speed, 10μm 40% 60% 80% 100% 20μm 30μm 40μm

8.Working with the Instrument

A few rules:

•Always begin at a low extraction level, then slowly increase it.

•Do not use high extraction levels unless absolutely necessary.

•Different specimen sizes require different extraction levels.

•The faster the trimming or sectioning speed, the lower the extraction

level should be.

•The larger and/or thicker the specimen to be trimmed, the lower the extraction value.

•When section specimens with a diameter of 0.5 cm, the anti-roll guide stretches the section adequately. For larger specimens, we recommend using the vacuum function.

Trimming, 1 x 1cm specimen
Trimming thickness	Speed	Extract. value
10μm	40%	1.5
10μm	60%	1.5
10μm	80%	1
10μm	100%	1
20μm	40%	2
20μm	60%	2
20μm	80%	1.5
20μm	100%	1
30μm	40%	2.5
30μm	60%	2.5
30μm	80%	2
30μm	100%	1.5
40μm	40%	3
40μm	60%	3
40μm	80%	2.5
40μm	100%	2

Trimming, 1 x 1cm specimen
Trimming thickness	Speed	Extract. value
1μm - 5 μm	40%	2.5
	60%	2
	80%	1.5
	100%	1.5
10μm	40%	2.5
	60%	2
	80%	2
	100%	1.5

The values listed here are possible settings that depend on the tissue type, specimen size, section thick- ness and section speed, and are in- tended for orientation purposes only.

These settings can also vary when using different anti-roll plate!

46 Operating Manual V1.1 – 05/2007