Leica CM1950 manual Control panel 3 Motorized sectioning optional, Sectioning modes

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7.Instrument Controls

Control panel 3 – Motorized sectioning (optional)

Switching the sectioning mode ("CUT MODE") from continuous stroke ("CONT") to single stroke ("SINGLE") (active)

Motor speed controller (0-100%)

Hold the button to section at maximum speed.

Release the button to continue section- ing at the previously selected speed (see controller, above).

Switch from slow speed range to fast speed range: while switching on the in- strument, press and hold down the Vmax button.

Fig. 34

Speed ranges

Slow:

0-50 strokes/min

Fast:

0-85 strokes/min

Vmax:

85-90 strokes/min

Sectioning modes

If emergency stop has been activat- ed, the cutting mode must be select- ed again.

The red LED in the E-STOP field indicates either:

emergency-stop function active, or

footswitch dummy (optional footswitch) connected incorrectly or not at all.

The mechanical handwheel brake is enabled when the yellow LED is lit in the M-STOP field.

The handwheel must also be locked when working on the specimen head.

Press to brake the handwheel electronically (LED lit) – specimen stops in lower (6 o’clock) position.

Can be used in any position.

1.Press the buttons at the same time to start motorized sectioning.

2.To end the sectioning process, press RUN/STOP, ENABLE or BRAKE.

- Specimen head stops at bottom (for BRAKE, automatic electronic braking takes place).

3.For motorized sectioning, does not have to be unlocked; continue working by pressing both the RUN/STOP and ENABLE keys.

4.If, during manual work using handwheel, you have braked using BRAKE also use BRAKE to release!

The instrument can be used in manual and motorized modes. The following settings are available:

Single stroke (SINGLE) or continuous stroke (CONT.) in automatic mode, as well as

ROCK (sectioning using handwheel) in manual mode.

When switching the instrument on, no operating mode is active for safety reasons.

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Operating Manual V1.1 – 05/2007

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Contents Leica CM1950 Page Page Table of contents 12.2 11.112.1 12.3Qualification of Personnel Symbols Used in This Manual and Their MeaningImportant Information Designated UseGeneral Safety Instructions Safety and DesignUnpacking and Installation Opening the packaging Transport to the Installation Location Ramp assemblyRemoving the packaging Safety Features Microtome knivesKnife guard Securing/Locking the HandwheelCleaning, Disinfection Turning the Instrument Back On Handling Specimens Defrosting MaintenanceRemoval of the Microtome Technical Data Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 HzStandard Delivery Set of filters 5 piecesHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Right side InstallationSite Requirements Left sideTransport to the Site Assembling the Handwheel Centering the Handwheel Optional Locking/Unlocking the HandwheelEmergency-stop function active, or Existing power system Connection to Power Supply SystemOnly for instruments sold in Japan Selecting the frequency Installation Inserting the Section Waste Tray Installing the Storage Systems Optional For reasonsShelf, Movable Optional Section waste tray with the a Cutout a facing the userInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Instrument Controls Control Panel Fields on the CM1950 Control Panel Field12 o’clock Position 6 o’clock 20.0μm Retraction0μm 60.0μmMotion continues as long as the button is held Coarse feed functionsBrief press of the button retracts the specimen by 20μm Control panel 3 Motorized sectioning optional Sectioning modesDisinfection Duration 30min Duration 180 min Leica Cryofect is not available in all countriesWorking with the Instrument Turning the Instrument OnConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Cutting with Extraction Anti-roll Guide installed Shut off VAC LED in VAC button goes out10μm 40% 60% 80% 100% 20μm 30μm 40μm SpeedSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Working with the Instrument Problems During Work TroubleshootingProblem Causes Remedies Cryochamber Sections tear Alternately Longer sufficient Temperature Selection Chart in minus C Tissue type Chamber temperatureOptional Accessories Ordering InformationCryofect, VPE Leica Cryofect 4x 350ml Lever 6 for the lateral shift When using low-profile blades, the rulerWith anti-roll guide Lever 7 for clamping the bladeBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Cleaning the knife holder CE Daily cleaning Unscrew the bolts 35 of the pressure plateNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories Maintenance and Cleaning General MaintenanceChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp NB Microtome knives must be in boxes This equipment has been cleaned and decontaminatedDecontamination Certificate master Yes No indicated belowBU Return Authorization Number Anne De Greef-Safft President Biosystems Division EC Declaration of ConformityWarranty and Service Peoples Republic of China