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| 9. Troubleshooting |
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Problem | Causes | Remedies |
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Ridged sections | Knife damaged. | Use different part of the knife. |
| Edge of | Replace |
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Chatter during sectioning | Specimen insufficiently frozen onto | Refreeze specimen onto the disc. |
| the specimen disc. |
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| Specimen disc not clamped tightly. | Check disc clamping. |
| Knife not clamped tightly enough. | Check knife clamping. |
| Specimen has been sectioned too | Refreeze specimen onto the disc. |
| thickly and has detached from disc. |
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| Very hard, inhomogenous specimen. | Increase section thickness; reduce |
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| specimen surface area if ecessary. |
| Blunt knife. | Use different part of the knife. |
| Knife profile inappropriate for | Use knife with different profile. |
| specimen cut. |
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| Incorrect clearance angle. | Set correct angle. |
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Condensation on
Brush, forceps and/or cloth too warm. | Store all tools on storage shelf in |
| the cryochamber. |
Replace | ||
after adjustment | edge. The adjustment was carried out | Be more careful next time! |
| in the direction of the knife. |
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Temperature incorrect for the | Select correct temperature. | |
| tissue cut. |
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| Knife profile inappropriate for the | Use knife with different profile |
| specimen cut. | (c or d). |
| Ice buildup on the knife back. | Remove ice. |
| Handwheel speed not uniform. | Adapt speed. |
| Knife not clamped tightly enough. | Check knife clamping. |
| Specimen disc not clamped tightly | Check disc clamping. |
| enough. |
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| Cryocompound applied to cold | Apply cryocompound to warm disc, |
| specimen disc; specimen detached | mount specimen and freeze. |
| from disc after freezing. |
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Leica CM1950 Cryostat | 49 |