Leica CM1950 manual Temperature Selection Chart in minus C, Tissue type Chamber temperature

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10.Temperature Selection Chart (in minus °C)

Tissue type

Chamber temperature

Specimen head temperature

Spleen

-15°C to -20°C

-11°C

 

 

 

Liver

-10°C

-20°C

 

-15°C

off to -15°C

 

 

 

Intestine

-10°C

-20°C

 

-15°C

A*: off to -20°C

 

 

E*: -20°C

 

 

 

Heart

-10°C

A: -20°C

 

 

E: -20°C to -30°C

 

-15°C

off to -20°C

 

 

 

Ovaries

-10°C

E: -20°C

 

-15°C

off to -15°C

 

 

 

Fallopian tubes

-10°

E: -20°C

 

-15°C

off to -15°C

 

 

 

Kidney

-10°C

-20°C

 

-15°C

A: off to -15°C

 

-20°C

-20°C

 

 

 

Muscle

-18°C to -20°C

-15°C

 

 

 

Skin with fat

-19°C

-32°C to -40°C

 

 

 

Hard fat

-19°C

-21°C to -25°C

 

 

 

Stomach

-10°C

-20°C

 

-15°C

off to -15°C

 

 

 

Brain

-15°C

-10°C, *E

 

 

 

*A = blocked, *E = complete

The temperature values given above are based on long-term experience, however, these are only approximate values, as any tissue may require particular adjustments.

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Operating Manual V1.1 – 05/2007

Image 52
Contents Leica CM1950 Page Page Table of contents 11.1 12.112.2 12.3Symbols Used in This Manual and Their Meaning Important InformationQualification of Personnel Designated UseGeneral Safety Instructions Safety and DesignUnpacking and Installation Opening the packaging Transport to the Installation Location Ramp assemblyRemoving the packaging Safety Features Microtome knivesKnife guard Securing/Locking the HandwheelCleaning, Disinfection Turning the Instrument Back On Handling Specimens Defrosting MaintenanceRemoval of the Microtome Technical Data Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 HzStandard Delivery Set of filters 5 piecesHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Installation Site RequirementsRight side Left sideTransport to the Site Assembling the Handwheel Centering the Handwheel Optional Locking/Unlocking the HandwheelEmergency-stop function active, or Existing power system Connection to Power Supply SystemOnly for instruments sold in Japan Selecting the frequency Installation Installing the Storage Systems Optional For reasons Shelf, Movable OptionalInserting the Section Waste Tray Section waste tray with the a Cutout a facing the userInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Instrument Controls Control Panel Fields on the CM1950 Control Panel Field12 o’clock Position 6 o’clock Retraction 0μm20.0μm 60.0μmMotion continues as long as the button is held Coarse feed functionsBrief press of the button retracts the specimen by 20μm Control panel 3 Motorized sectioning optional Sectioning modesDisinfection Duration 30min Duration 180 min Leica Cryofect is not available in all countriesWorking with the Instrument Turning the Instrument OnConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Cutting with Extraction Anti-roll Guide installed Shut off VAC LED in VAC button goes out10μm 40% 60% 80% 100% 20μm 30μm 40μm SpeedSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Working with the Instrument Problems During Work TroubleshootingProblem Causes Remedies Cryochamber Sections tear Alternately Longer sufficient Temperature Selection Chart in minus C Tissue type Chamber temperatureOptional Accessories Ordering InformationCryofect, VPE Leica Cryofect 4x 350ml When using low-profile blades, the ruler With anti-roll guideLever 6 for the lateral shift Lever 7 for clamping the bladeBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Cleaning the knife holder CE Daily cleaning Unscrew the bolts 35 of the pressure plateNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories Maintenance and Cleaning General MaintenanceChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp This equipment has been cleaned and decontaminated Decontamination Certificate masterNB Microtome knives must be in boxes Yes No indicated belowBU Return Authorization Number Anne De Greef-Safft President Biosystems Division EC Declaration of ConformityWarranty and Service Peoples Republic of China