Leica CM1950 manual Cleaning, Disinfection Turning the Instrument Back On

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2.Safety and Design

2.5Cleaning, Disinfection – Turning the Instrument Back On

It is not necessary to remove the microtome for disinfection.

•The instrument has been designed for UV disinfection! Spray disinfec- tion with Leica Cryofect is also possible, thanks to the special insulation of the microtome. (Cryofect is not available in all countries!)

Remove section waste after EVERY sectioning operation and BE- FORE changing specimens. Remove the section waste using a pa- per towel soaked in Cryofect or an alcohol-based disinfectant) or remove the waste using the extraction nozzle (optional). Do not start the disinfection until after folding the section stretcher to the side! Each new specimen is a potential source of contamination.

•When disinfecting the instrument, take appropriate protective measures (gloves, mask, protective clothing, etc.).

•When using detergents and disinfectants please comply with the safety precautions of the disinfectant manufacturer.

•The integrated glass anti-roll guide of the blade holder CE, CN and CN-Z can be cleaned either with acetone or alcohol.

•Dispose of waste liquid according to the waste disposal regulations!

•Do not use external heaters for drying the cryochamber. This can cause damage to the cooling system!

•Do not turn the instrument on before the cryochamber is completely dry! Frost formation!

•All components removed from the cryostat must be carefully dried be- fore returning them to the cryochamber!

•The front panel and the slit cover of the microtome must be completely dry before turning on the instrument!

For more detailed information about disinfection, visit the Leica

Microsystems website at

www.histo-solutions.com

12	Operating Manual V1.1 – 05/2007

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Contents Leica CM1950 Page Page Table of contents 11.1 12.112.2 12.3Symbols Used in This Manual and Their Meaning Important InformationQualification of Personnel Designated UseSafety and Design General Safety InstructionsUnpacking and Installation Opening the packaging Ramp assembly Transport to the Installation LocationRemoving the packaging Safety Features Microtome knivesKnife guard Securing/Locking the HandwheelCleaning, Disinfection Turning the Instrument Back On Maintenance Handling Specimens DefrostingRemoval of the Microtome Technical Data Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 HzStandard Delivery Set of filters 5 piecesHandwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Installation Site RequirementsRight side Left sideTransport to the Site Assembling the Handwheel Locking/Unlocking the Handwheel Centering the Handwheel OptionalEmergency-stop function active, or Connection to Power Supply System Existing power systemOnly for instruments sold in Japan Selecting the frequency Installation Installing the Storage Systems Optional For reasons Shelf, Movable OptionalInserting the Section Waste Tray Section waste tray with the a Cutout a facing the userInstalling the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Instrument Controls Control Panel Fields on the CM1950 Control Panel Field12 o’clock Position 6 o’clock Retraction 0μm20.0μm 60.0μmCoarse feed functions Motion continues as long as the button is heldBrief press of the button retracts the specimen by 20μm Control panel 3 Motorized sectioning optional Sectioning modesDisinfection Duration 30min Duration 180 min Leica Cryofect is not available in all countriesWorking with the Instrument Turning the Instrument OnConfiguring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Cutting with Extraction Anti-roll Guide installed Shut off VAC LED in VAC button goes outSpeed 10μm 40% 60% 80% 100% 20μm 30μm 40μmSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Working with the Instrument Troubleshooting Problems During WorkProblem Causes Remedies Cryochamber Sections tear Alternately Longer sufficient Temperature Selection Chart in minus C Tissue type Chamber temperatureOptional Accessories Ordering InformationCryofect, VPE Leica Cryofect 4x 350ml When using low-profile blades, the ruler With anti-roll guideLever 6 for the lateral shift Lever 7 for clamping the bladeBlade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Cleaning the knife holder CE Daily cleaning Unscrew the bolts 35 of the pressure plateNot Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories Maintenance and Cleaning General MaintenanceChanging Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp This equipment has been cleaned and decontaminated Decontamination Certificate masterNB Microtome knives must be in boxes Yes No indicated belowBU Return Authorization Number Anne De Greef-Safft President Biosystems Division EC Declaration of ConformityWarranty and Service Peoples Republic of China