Dynex 91000051 manual Pump Calibration, Calibration Protocol

Pump Calibration

Pump Calibration

Each Opsys MW™ washer manifold, or head, (e.g., 1 x 8, 1 x 12) has three calibration values stored in memory. These values are calculated and stored during manufacture at DYNEX Technologies. When the Opsys MW configuration is changed from one manifold to another, the corresponding calibration settings will be recalled by correctly setting the head size using the SETUP/HEAD feature (refer to page 28 for Specifying the Wash Head).

This calibration protocol may be used at any time to verify the performance of the Opsys MW washer.

IMPORTANT! It is recommended that this protocol be completed if any fluid-carrying component of the Opsys MW is replaced. This protocol may also be used to recalibrate the Opsys MW if a new wash buffer with different viscosity is used. Customize the protocol to emulate your assay, using the appropriate dispense volume, pump speed and fluid density.

Calibration Protocol:

1.Define a flat bottom plate profile using the SETUP/PLATE function (refer to page 30 for Specifying Plate Parameters).

2.Program an assay to dispense 200µl/well into columns 1-12 (refer to page 41 for Creating a New Wash Protocol).

3.Obtain a clean, dry flat bottom 96-well styrene plate.

4.Weigh the plate and record the empty plate weight in grams.

5.Run the assay programmed in Step 2. (This assay will dispense 200µl to all 96 wells in the plate.)

6.Weigh the plate and record the filled plate weight in grams.

7.Calculate the actual dispensed volume and % error using the following formulas:

A.Actual Volume = [(Full weight – Empty weight) x 1000mg/g] / (96 wells x fluid density)

B.%Error = 100 x (Actual Volume – Target Volume)/Target Volume

Example:

Empty plate weight: 48.38g

Full plate weight: 67.42g

Density of water at 20°C = 0.998mg/µl

Actual Volume = [(67.42 – 48.38) x 1000]/(96 x 0.998) = 198.7µl/well

%Error = 100 x (198.7 – 200)/200 = -0.6%

74 Opsys MW™ User’s Guide