Wash Protocols Wash Protocol Operations, Purge | Dynex 91000051

Wash Protocol Operations

Wash Protocol Operations

Chapter 4 Wash Protocols

Wash Protocol Operations

Awash protocol consists of a series of Purge, Move, Soak, Aspirate, Dispense and Fill operations.

Purge, Move and Soak can be carried out in any sequence. Aspirate, Dispense and Fill can only be carried out within a Move operation, and there cannot be a Move within a Move.

Each of these operations is described below:

	Operation		Description
			Dispenses fluid from the dispense wash pins while

	Purge
			the wash head is positioned over the purge tray
			Note: Purge is usually
			carried out at the beginning
			of a wash protocol to rinse
			the dispense wash pins and
			remove air bubbles.
	Move		Performs Aspirate, Dispense, Fill and/or Soak
	Move		Performs Aspirate, Dispense, Fill and/or Soak
			operations on specified strips of the Microplate
	Aspirate		Removes the contents of a well by positioning the
			wash pins at the aspiration height in the well and
			aspirating the liquid from the wells
			A sweep may also be performed during Aspirate.
			Note: The aspirate height
			and sweep parameters for a
			particular plate type are
			specified during system
			configuration (30).
			If a sweep is specified during
			configuration, the wash pins
			are moved back and forth
			during the aspiration cycle.

Opsys MW™ User’s Guide

39

Image 47

Dynex 91000051 manual Wash Protocols Wash Protocol Operations, Purge, Move, Performs Aspirate , Dispense , Fill and/or Soak

Contents

User’s Guide Please read carefully before using the Opsys MWOpsys MW Microplate Washer Chantilly, VA 20151-1683 USA Tel. 703 800 288-2354 Fax. 703 Part No. 91000051, Revision BThis manual is published by DYNEX Technologies, Inc DYNEX Technologies 14340 Sullyfield Circle Warranty and Special Provisions Limited Warranty Page Chapter 3 Configuration Table of ContentsTable of Contents Chapter 2 Installation Opsys MW User’s Guide Chapter 7 TroubleshootingChapter 8 Service Index This manual gives you the information needed to About this ManualAbout this Manual This page is intentionally left blank Description Chapter 1 IntroductionDescription Programming Description Wash HeadWash Head Dispense Height Wash Head PositionsWash Head Position Description Waste Wash Fluid Opsys MW User’s Guide Features Features Opsys MW User’s Guide Specifications SpecificationsDimensions and Weight Power Requirements Assay Programming Input RangesStandards Plate Setup Label Description Warning LabelsWarning Labels Opsys MW User’s Guide Unpacking Chapter 2 Installation UnpackingMaterials Provided To unpack the components Hardware Components Hardware Components Keypad. The keypad is used by the operator to select commands and enter wash parameters Figure 3. Rear View of the Opsys MW System Connectors at the rear of the Opsys MW system are shown in Figure Positioning the Instrument Positioning the Instrument Installing the Wash Head Installing the Wash Head Installing the Wash Container Installing the Wash ContainerTo install the Wash Container Installing the Waste Container Installing the Waste ContainerTo install the Waste Container and Foam/Aerosol Trap 1. Connect the Opsys MW to the laboratory electrical supply outlet Turning on the SystemTurning on the System SETUP PROGRAM 7 8 9 Chapter 3 Configuration The KeypadThe Keypad If you are entering or editing information , a “Cancel” keyPurpose Scroll keys pressing the key once. If the key is pressed two to AlphanumericKeys The numeral shown on the key is displayed by Menus SETUPConfigure the Instrument Menus The PROGRAM Menu The SETUP Menu CLEAN Configuring the SystemConfiguring the System Command Specifying the Wash Head Specifying Plate Parameters To specify parameters for a plate type Note The default value is displayed. Select SHOW to move the wash head to the default well top position so that the height can be checked. Then, determine the correct value as outlined on page 30. Repeat the procedure as needed Sweep height? SHOW Opsys MW User’s Guide Displaying the Software Version Note Refer to page 74 for additional pump calibration instructions Specifying the Pump Parameters Selecting Clean Opsys MW User’s Guide Purge Chapter 4 Wash Protocols Wash Protocol OperationsWash Protocol Operations Operation Fill DispenseSoak Creating a New Wash Protocol Creating a New Wash ProtocolSpecifying the Header To create a new wash protocol header 9. If YES is selected, the display will read Constant timing? YESNO 7. Re-enter the password and press ENTER. The display will readSelect plate 2 DYNEX1 Variable strips? YESNO Select option MOVE PURGE Specifying Wash Cycle Operations To specify a Purge To specify a Move cycle Note An Aspirate or Dispense must be To specify an Aspiration within a Move Note A Dispense or Aspirate must be performed within a Move cycle To specify a Dispense within a Move Opsys MW User’s Guide To specify a Soak within a Move Note A Fill must be performed within a Move cycle To specify a Fill within a MoveNote A volume ranging from 50 to 300 microliters can be entered Select option END LOOP 3. Select END LOOP. The display will read Ending a MOVE CycleSelect option SOAK FILL 2. Scroll right to display the next selection. The display will read To specify a Soak outside of a Move Select option SOAK END 2. Select END. The display will read 1 NAME01 To specify the End of the wash protocol Modifying a Wash Protocol Modifying a Wash ProtocolTo modify a wash protocol MOVE To modify a wash operation To delete a wash operation To insert a wash operation Copying a Wash Protocol Copying a Wash ProtocolTo copy a wash protocol Overwrite test? NO YESNO 8. Select YES. The display will read 7 NAME04 SETUP PROGRAM Preparation Chapter 5 Washing a PlatePreparation To empty the waste container To refill the wash container Washing a Plate Washing a PlateTo wash a plate Opsys MW User’s Guide Six month maintenance Chapter 6 Routine Maintenance Routine Maintenance ProceduresDaily maintenance Weekly maintenance As required maintenance To decontaminate the system Cleaning and DecontaminationTo clean the system Cleaning and Decontamination Cleaning the Containers Cleaning the ContainersTo empty and clean the waste container Replacing the Tubing Replacing the TubingTo replace the dispense tubing To replace the vacuum tubing To replace the waste tubing Pump Calibration Pump CalibrationCalibration Protocol 8. If the dispensed volume is too high, decrease the pump speed using the SETUP/CAL function refer to page Opsys MW User’s Guide Resolution Chapter 7 Troubleshooting Operational ProblemsOperational Problems Symptom Probable Cause waste container is Test Too Large is High Waste LevelX Motor Home Z Motor Home To replace the wash head assembly Chapter 8 Service Service ProceduresTo remove the wash head assembly To clean the wash head assembly Hardware AccessoriesSpare Parts Major Assemblies Returning a Washer for Service Returning a Washer for ServiceTo return a Washer for service Suggested decontamination methods EQUIPMENT IN TRANSITEQUIPMENT DECLARATION CERTIFICATE OF DECONTAMINATION DYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151 USA Contact DYNEX TechnologiesContact DYNEX Technologies Index Index variable strips, 41 warning labels, 11 Wash Container, 5