Dynex 91000051 manual Wash Protocols Wash Protocol Operations, Purge, Move, Aspirate, Description

Wash Protocol Operations

Chapter 4 Wash Protocols

Wash Protocol Operations

Awash protocol consists of a series of Purge, Move, Soak, Aspirate, Dispense and Fill operations.

Purge, Move and Soak can be carried out in any sequence. Aspirate, Dispense and Fill can only be carried out within a Move operation, and there cannot be a Move within a Move.

Each of these operations is described below:

Operation	Description
	Dispenses fluid from the dispense wash pins while
Purge
	the wash head is positioned over the purge tray
	Note: Purge is usually
	carried out at the beginning
	of a wash protocol to rinse
	the dispense wash pins and
	remove air bubbles.
Move	Performs Aspirate, Dispense, Fill and/or Soak
	operations on specified strips of the Microplate
Aspirate	Removes the contents of a well by positioning the
	wash pins at the aspiration height in the well and
	aspirating the liquid from the wells
	A sweep may also be performed during Aspirate.
	Note: The aspirate height
	and sweep parameters for a
	particular plate type are
	specified during system
	configuration (30).
	If a sweep is specified during
	configuration, the wash pins
	are moved back and forth
	during the aspiration cycle.

Opsys MW™ User’s Guide 39