Dynex 91000051 Operational Problems, Symptom, Probable Cause, Resolution, waste container is

Operational Problems

Symptom	Probable Cause	Resolution
Dispense volumes	The dispense	Check the pins, seals and
are incorrect.	pins are leaking.	tubing for leaks.
	The dispense	Check the seals and
	pressure is low.	tubing for leaks.
		Check for air leaks.
		Reseat the tubing in the
		dispense valve.
	The dispense	Remove the wash head
	pins are clogged.	assembly and clean it
		using the manifold head
		cleaning wire. Then run a
		purge. Service is required
		if problem persists.
	Incorrect	Recalibrate Pump
	Calibration
Aspiration is not	The aspiration	Remove the wash head
complete.	pins are clogged.	assembly and clean it
		using the manifold head
		cleaning wire. Service is
		required if problem
		persists.
	The aspiration pin	Repeat the plate setup
	height is set too	procedure (page 30).
	high.
	The seal on the	Check the seal and waste
	waste container is	tubing for leaks.
	leaking.
	The wash	If the bottoms of the plate
	protocol is not	wells are flat, use a
	optimized.	sweep.
		Add additional aspiration
		cycles to the wash
		protocol.
	Aspiration tubing	Check the aspiration
	may be blocked	tubing and remove any
	or kinked.	blockages or kinks.
	The vacuum	Remove any obstructions
	exhaust may be	at the vacuum exhaust
	blocked.	(see page 17).
	The vacuum	Service is required.
	pump may be
	contaminated by
	overflow.

Opsys MW™ User’s Guide 79