Advanced Operations
Option 2:
1.Insert the blank cuvette/cell into the cuvette/sample cell holder. Press Zero.
2.Insert the sample cuvette/cell into the cuvette/sample cell holder. Press Read.
•The newly plotted wavelength scan curves are shown in black.
•The absorbance or transmittance value and the associated wavelength are highlighted in black.
3.Press Options and then Reference: Off in the Options menu to select another scan to display on the same screen with the current scan. Highlight the required scan number and press Highlight Reference.
Note: After selecting a reference scan the Reference: Off key in the
Options menu turns into Reference: On.
Note: Only scans that have the same wavelength range and step can be displayed using the overlay option. A reference curve can only be displayed with another active curve. Two previously stored scans cannot be overlaid.
4.The reference curve is shown in gray. The absorbance or transmittance value and the associated wavelength are highlighted in gray.
•Additionally the difference of the extinction and/or transmission value between the two Scans (measured Scan and reference Scan) is indicated/highlighted at each position of the cursor.
Note: A black and an gray box are shown in the left corner of the display. The gray box relates to the reference scan and the black one relates to the current wavelength scan.
5.Press the black or gray small box in the left upper corner on the screen to switch between the actual wavelength scan and reference scan.