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Advanced Operations
6.4Single Wavelength (absorbance, concentration and transmittance measurements)
The Single Wavelength mode can be used in three ways. For sample measurements at a single wavelength, the instrument can be programmed to measure the absorbance, % transmittance or concentration of the analyte.
Absorbance measures the amount of light absorbed by the sample, in units of absorbance.
%transmittance measures the percent of the original light that passes through the sample and reaches the detector.
Turn the concentration factor on to select a specific multiplier for converting absorbance readings to concentration. In a graph of concentration versus the absorbance, the concentration factor is the slope of the line.
6.4.1 Set up single wavelength mode
Press Single Wavelength in the Main Menu. Press Options for
Parameter Setup.
| Table 12 Single wavelength setup options | |
Options | Description | |
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More | For further Options | |
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Store Off/On | With the Store On setting, all measurement data are stored automatically. With the Store Off | |
setting, no measurement data are stored. | ||
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% Trans/Abs | To switch to % transmittance, concentration or absorbance readings | |
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λ Wavelength | To enter the measurement wavelength. Use the alphanumeric keypad to enter the measurement | |
wavelength. The entered wavelength must be in the range from | ||
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| This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed | |
Timer icon | (e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has | |
elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement | ||
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| program. | |
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Concentration Factor | Multiplication factor for converting absorbance values into concentration values. | |
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Concentration | To select the position of the decimal point in the calculated concentration readings. | |
Resolution | ||
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