Hach DR 5000 user manual Set up single wavelength mode, Single wavelength setup options, Program

Advanced Operations

6.4Single Wavelength (absorbance, concentration and transmittance measurements)

The Single Wavelength mode can be used in three ways. For sample measurements at a single wavelength, the instrument can be programmed to measure the absorbance, % transmittance or concentration of the analyte.

Absorbance measures the amount of light absorbed by the sample, in units of absorbance.

%transmittance measures the percent of the original light that passes through the sample and reaches the detector.

Turn the concentration factor on to select a specific multiplier for converting absorbance readings to concentration. In a graph of concentration versus the absorbance, the concentration factor is the slope of the line.

6.4.1 Set up single wavelength mode

Press Single Wavelength in the Main Menu. Press Options for

Parameter Setup.

	Table 12 Single wavelength setup options
Options	Description
More	For further Options
Store Off/On	With the Store On setting, all measurement data are stored automatically. With the Store Off
	setting, no measurement data are stored.
% Trans/Abs	To switch to % transmittance, concentration or absorbance readings
λ Wavelength	To enter the measurement wavelength. Use the alphanumeric keypad to enter the measurement
	wavelength. The entered wavelength must be in the range from 190–1100 nm.
	This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed
Timer icon	(e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has
	elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement
	program.
Concentration Factor	Multiplication factor for converting absorbance values into concentration values.
Concentration	To select the position of the decimal point in the calculated concentration readings.
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