Complete a standard addition, Sample volume | Hach DR 5000 specs

Advanced Operations

e.Does the test require the sample to be at a certain temperature?

f.Was the pH of the sample in the correct range?

g.Is the pipette volume correct?

2.Check the used reagents by repeating the standard addition procedure with freshly prepared reagents.

	Detection rate	Conclusion

		The originally used reagents were defective.
	100%	Check the standard solution:
	100%	Repeat the standard addition procedure with a
		Repeat the standard addition procedure with a
		freshly prepared standard solution.

		Detection rate	Conclusion

			The originally used
		100%	standard solution was
			defective.

If none of these measures resolves the problem, please contact the manufacturer or a sales representative.

6.3.1 Complete a standard addition

Carry out the standard addition in accordance with the corresponding procedure.

There are two different methods:

Peak volume (Standard addition):

Defined volumes of a standard solution are added step by step to an already analyzed sample. The sample is measured again after each addition.

Sample volume:

A defined volume of a standard solution of known concentration is added to the volume of sample specified in the procedure and the sample is measured after each addition. In most cases three different standard solutions are prepared and the procedure is repeated for each of them.

Note: The units and chemical forms used for the sample are used for the standard solutions. Be careful to ensure that to use the correct units for subsequent entries.

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Hach DR 5000 Complete a standard addition, Peak volume Standard addition, Sample volume, Check the standard solution

Contents

January 2008, Edition Page Table of Contents Table of Contents 101 Table of Contents Section Specifications Specifications Use of hazard information Safety informationPrecautionary labels General Information General Information Class 1 Laser Chemical and Biological SafetySource Lamp Safety General Information Overview of product General Information Operating environment Section InstallationUnpack the instrument Connection Installation Cell compartment Multi Cell Holder Top and Bottom 1 100 mm adapter optional Installation Multi Cell Holder Installation Installation Multi Cell Holder Beam path Beam path Language selection Power the instrument on and offChanging the language setting Start Up Start Up Use of the alphanumeric keypad Standard OperationsOverview Tips for the use of the touch screen Main Menu options Standard Operations Main Menu Operator ID Instrument Setup modeStandard Operations Standard Operations Sample ID Display and sound preferences Date and time VIS-Lamp and UV-Lamp Lamp Control Effect caused by the lamp settings on the Measuring Mode Standard Operations Keyboard Printer setupUSB connector Standard Operations PC and printer Printer Setup Hach Data Trans Print data Password Password deactivation Standard Operations Recall stored data from the data log Store, recall, send and delete dataData Log Auto/manual data storage To send measurement data to a PC Send data from the data log Delete stored data from the data log Select Wavelength Scan or Time Course to recall data Data storage from wavelength scan or time courseRecall stored data from wavelength scan or time course Send data from wavelength scan or time course Option Press Wavelength Scan or Time Course and then Delete stored data from wavelength scan or time course Stored programs options Stored ProgramsSelect a saved test/method enter user-specific basic data Stored program options Options Description Store Off/On Timer iconReading Mode Standard Adjust Set the reading mode Standard Operations Use of program timers Press OptionsMore…Dilution Factor Set the dilution factorTake single wavelength measurements single reading Take single wavelength measurements continuous readings Run a standard adjust Press OptionsMoreChemical Form Change of the default setting of the chemical formPress Save as Default Set the chemical form Press OptionsMoreReagent Blank Standard Operations Run a reagent blankMeasurement/analysis of a reagent blank Add stored programs to the favorite programs list Standard Operations Analysis of samples Cell compartment #1 Barcode Programs Select the chemical evaluation form Changing the standard settingStandard Operations Complete a barcode 13 mm test/vial Select the measuring range Barcode program options Basic test-specific and sample-specific data settings Edit Sample blankOptions Description Send Data icon Enter the Name Update/edit barcode testsManual update of a barcode test Program update options Enter the Measurement Process Enter the Version Timer 1, Timer 2, Timer 3 and Timer Press OptionsMoreEdit Update an existing barcode testOption 1 Manual update of test data Option 2 Manual check/revision of test data Program a new test Upgrade of the instrument software Press OK to return to the System Checks menu Standard Operations Program a user method Advanced OperationsUser Programs Program Type Advanced OperationsProgram Number Program Name Wavelength single wavelength program type Single wavelength settingsConcentration resolution number of decimal places Units Absorbance formula multi-wavelength program type Multi wavelength settingsChemical form Concentration Factor Kx List of available absorbance formulasWavelength λx Calibration by entering calibration values Calibration settings for single and multi wavelength modeCalibration settings Advanced Operations Advanced Operations Advanced Operations Upper and lower limits of the measuring range Store a user programAdditional user-defined parameters and functions Chemical form 2 / chemical form 3 / chemical form Timer 1 / Timer 2 / Timer 3 / Timer Definitions of the program points Advanced Operations Free programming program typeMeasurement process Enter a new element of a measuring sequence Content and definition of the keys Key / Zeroing Key / Reading Process Timer key Enter calibration formula C1 Enter the calibration formula evaluation formulaDelete an element of a measuring sequence Insert an element of a measuring sequence New Number Edit formula key descriptionsScreen Key Description C2 On Enter the next calibration formula C2 or Cn Select Conc. key Enter variablesSave a free programming user program 4 Add, edit and delete user programs from the favorites list Advanced Operations Select a user program Delete Add to FavoritesEdit Delete a favorite program Favorite ProgramsRecall a favorite program Method Measures to identify other interference factors ChecklistStandard Addition monitoring/checking results Complete a standard addition Peak volume Standard additionSample volume Check the standard solution Press OptionsMoreStandard Addition Peak volume/sample volume methods Description of the table of readings Advanced Operations Program Set up single wavelength modeSingle wavelength setup options Options Description Concentration resolutionConcentration factor Take single wavelength measurements continuous readings Reading modeTake single wavelength measurements single reading Multi-wavelength setup options Set the reading mode at different wavelengths Absorbance formula Press Absorbance Formula Concentration factor For data storage, see .3.1.1 on Wavelength scan setup options Set up the wavelength scan Scale & Units Setting wavelengthOption Description Integral Cursor modeSelect view displaying table Scale & units Press Scale & Units Wavelength scan reading Navigating the wavelength scan Highlight Reference Advanced Operations Work with reference scans 100 101 Time course setup parametersTime course setup options Time course of absorbance/transmittance 102 Time course scan readingTime & interval Scale & units 103 Analysis of time course data 104 Navigation of a time scan or a time scan analysisNavigating the time scan 105 System checksInstrument information 106 Advanced Operations Upgrade of the instrument softwareOptical checks 107 Wavelength check 108 Noise check 109 110 Stray light check 111 Absorbance check 112 Drift check Press Nominal Values 113 Verification kit 114 Press Verification 115 Output checksLamp history 116 Advanced Operations Factory serviceService time 117 Instrument Backup 118 Restore backup data 119 120 Display MaintenanceCleaning requirements Spectrophotometer 122 Lamp replacementMaintenance Tungsten lamp replacement 123 Deuterium lamp UV replacement 124 Fuse replacement 125 Filter pad maintenanceFilter pad replacement 126 Base of DR 5000 with filter grid 127 Section Troubleshooting 128 Troubleshooting Description Cat.-No Section Replacement Parts129 130 Replacement Parts 131 Contact Information 132 Contact Information 133 Limited WarrantyLimitations Limitation of Remedies 134 Limited Warranty 135 Index 136