BioOptix™ 10

Section 3 Operation

3.3 BioOptix 10 Software

 

 

 

Note

 

 

 

 

Refer to On-line Help for specific BioOptix 10 software operat-

 

 

 

 

ing instructions.

3.3.1 Overview

The operation of the BioOptix 10 system is directed by three

 

 

files:

 

 

 

• Gradient – these files define how a gradient is to be

 

 

 

formed from Buffer A and B solutions.

 

 

 

• Method – these files contain the settings that direct the

 

 

 

operation of each channel of the system. A method file

 

 

 

will reference the settings in a gradient file.

 

 

 

• Experiment – These files contain settings that direct the

 

 

 

operation of all channels. Experiment files are based on

 

 

 

method file settings. The BioOptix 10 system operation

 

 

 

can be started after the experiment settings are defined.

 

 

Sections 3.4 through 3.6 describe these files in detail.

3.4 Gradients

A gradient file can be created by:

 

 

 

• selecting the File>New>Gradient menu command,

 

 

 

• pressing the Ctrl+G keyboard buttons,

 

 

 

• or by clicking the G toolbar button.

 

 

A new gradient file displays an empty two-column table and a

 

 

plot area (Figure 3-1). The table contains inflection points —

 

 

 

points at which the rate of gradient formation change.

 

 

The Initial volume row in the table defines the starting mixture,

 

 

expressed as percent B.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Figure 3-1 Gradient File

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Teledyne manual BioOptix 10 Software, Gradients, Overview