Teledyne manual BioOptix 10 Software, Gradients, Overview

BioOptix™ 10

Section 3 Operation

3.3 BioOptix 10 Software

		Note
		Refer to On-line Help for specific BioOptix 10 software operat-
		ing instructions.
3.3.1 Overview	The operation of the BioOptix 10 system is directed by three
	files:
		• Gradient – these files define how a gradient is to be
		formed from Buffer A and B solutions.
		• Method – these files contain the settings that direct the
		operation of each channel of the system. A method file
		will reference the settings in a gradient file.
		• Experiment – These files contain settings that direct the
		operation of all channels. Experiment files are based on
		method file settings. The BioOptix 10 system operation
		can be started after the experiment settings are defined.
	Sections 3.4 through 3.6 describe these files in detail.
3.4 Gradients	A gradient file can be created by:
		• selecting the File>New>Gradient menu command,
		• pressing the Ctrl+G keyboard buttons,
		• or by clicking the G toolbar button.
	A new gradient file displays an empty two-column table and a
	plot area (Figure 3-1). The table contains inflection points —
		points at which the rate of gradient formation change.
	The Initial volume row in the table defines the starting mixture,
	expressed as percent B.

Figure 3-1 Gradient File

3-2