BioOptix™ 10

Section 3 Operation

3.6.3Saving the Experiment Save the experiment by selecting the File>Save menu command.

File

Use a descriptive file name so that it can be located for later use.

3.7 Running the

With the experiment file open, some BioOptix 10 operation

Experiment

buttons along the bottom of the window are active (Figure 3-8).

 

Buttons will be active or inactive according to the current state of

 

the system.

 

 

 

 

 

Fast

Next

 

 

Play

Stop Forward

Tube

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Figure 3-8 BioOptix 10 Operation Buttons

 

Table 3-1 Operation Buttons

 

 

 

 

Button

Description

 

Play

Click to begin the experiment run. If the Stop

 

 

button was clicked during the run, clicking the

 

 

Play button will resume the run.

 

 

 

 

Stop

Click to suspend the run activity. From this state

 

 

you click Play to resume the run, or click Fast

 

 

Forward to skip to the next block or abort the

 

 

run.

 

 

 

 

Fast Forward

Click the Fast Forward to skip to the next block

 

 

or abort the run.

 

 

 

 

Next Tube

Click this button to advance the fraction collector

 

 

to the next row. This is useful when there is a

 

 

need to isolate effluent.

 

 

 

 

AutoZero

The baseline absorbance trace may shift if

 

 

buffer solutions are changed mid-run. Click this

 

 

button to re-zero the absorbance trace.

 

 

 

 

Show Run Window

Click this button to bring the experiment window

 

 

for the run in progress to the foreground. If you

 

 

have several other files (e.g. previous experi-

 

 

ments or new methods) open in the BioOptix 10

 

 

window this button lets you quickly return to the

 

 

running experiment.

 

 

 

3.7.1 System Operation

To start the experiment, click the Play button. The system begins

 

operation with the first block and proceeds according to the

 

experiment and method files.

Referring to Figure 3-7, this first block would be “Equilibrate” and use the operating parameters specified by the method file (Figure 3-2). The system would pump 0 %B (100% of buffer A) at

5.0mL/min. The system would pump 50 mL and then pause (Figure 3-9). Similarly, the BioOptix 10 would proceed through the Sample Load, Wash, Elute, and Flush blocks.

3-9

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Teledyne 10 manual Running, Experiment, Operation Buttons, File, System Operation