BioOptix™ 10

Section 3 Operation

3.Select a wash fluid. The wash fluid must be compatible with the buffer system and of sufficient strength to dis- solve salts. If this solution is different than the buffers, move the Buffer In lines to the wash fluid container.

4.For all selected channel positions, turn the Mode knob to the purge pump position.

5.Click the Start button to begin the flush routine. The BioOptix 10 flushes the pumps.

6.When the routine is finished, return the Mode knobs to the Operate position.

The flush routine can be modified to meet your application’s needs. To modify the pump flush select the Tools>Edit Pump Flush menu command. This command opens a window that allows you to change the flush volume, flow rate, Buffer A and B mixing, and the stroke volume. Click the Save button to save your changes.

3.8.2 Saving Run Data

The BioOptix 10 software automatically saves the run data and

 

chromatograms using the experiment name as the filename. Two

 

types of files are saved: “.lst” and “.run” files.

 

This run data can be retrieved and viewed at a later time using

 

BioOptix 10 software. To do so, select the File>Open menu

 

command or click the Open toolbar button. Multiple channels

 

from the experiment can be opened by select the “.lst” file. Indi-

 

vidual channels can be viewed by opening a “.run” file.

 

Viewing the run data and chromatograms is very similar to the

 

operation displays described in section 3.7.2.

 

The run data and chromatograms displayed in the active window

 

may be exported in various formats for archiving and sharing.

 

• Hard copies can be produced by selecting the File>Print

 

menu command or clicking the Print toolbar button.

 

Before printing you can also change the page setup

 

(File>Page Setup…) and preview the page (File>Print

 

Preview).

 

• Other file formats can be produced using the File>Save

 

As menu command. These formats are:

 

DOC–Microsoft Word format that includes the run

 

data and chromatogram.

 

RTF–Rich Text Format that includes the run data

 

and chromatogram. This format can be opened with

 

many word-processing programs.

 

PDF–Portable Document Format that is an

 

electronic file similar in appearance to the hard copy

 

output. Viewing PDF files require Adobe’s Acrobat

 

Reader, a free download from www.adobe.com.

 

TXT–Text-only format that lists the run parameters

 

and history. This format does not support

 

chromatogram data.

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Image 48
Teledyne 10 manual Saving Run Data, Preview