BioOptix™ 10

Appendix B Fluid Path Diagrams

B.1 Fluid-type Blocks

This appendix contains simplified diagrams of the plumbing and valves internal to the BioOptix 10 system. Various operating states are depicted for a single channel. A complete plumbing diagram can be found in Figure B-7.

An understanding of fluids paths can be helpful when designing custom template files (Appendix A), manually controlling the system, or troubleshooting.

The valves in the diagrams are:

Buffer–This valve switches between Buffer A and Buffer B to produce the correct mix (%B) of buffer solution.

Sample–This valve allows the pump to draw either sample from the Sample In ports or buffer solution.

Channel–This valve controls allows fluid to be pumped only when the channel is active.

Mode–This manually-controlled front-panel valve has three positions:

Operate–passes fluid from the pump to the column.

Manual Inject–directs injected fluid onto the column.

Purge Pump–diverts fluid from the pump to a purge port that is common to all channels.

Effluent–This valve sends the effluent to the Foxy fraction collector or to the channel’s waste port.

Fluid blocks pump buffer solution through the column (Figure B-1).

 

To Pump Purge

Manual Sample Injection

 

Channel

 

Mode

 

 

 

Sample

 

 

 

Sample In

 

 

 

 

 

 

Column

Buffer

 

 

 

 

Pump

 

 

 

 

Effluent

Buffer A

Buffer B

To Waste

To Foxy

Figure B-1 Fluid path for Fluid-type blocks

B-1

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Teledyne 10 manual Appendix B Fluid Path Diagrams, Fluid-type Blocks