Teledyne 10 B.2 Load-type Blocks

BioOptix™ 10

Appendix B Fluid Path Diagrams

B-2

While the system is running a fluid-type block:

• The Buffer valve switches between buffe rs A and B to

produce the correct mix.

• The Sample valve allows only buffer solution to pass.

• If th e channel is active, the Channel valve allows the

fluid to be pumped. If the channel is inactive, the valve

prevents fluid from being pumped (Figure B-2).

• The Mode valve is in the Operate position.

• The Effluent valve can direct the fluid to waste or to the

fraction collector. Directing the effluent to waste is

practical when the fluid block is in the experiment for

column equilibration. Collecting the effluent may be

desirable if the fluid may contain something of interest

(e.g. during a column wash).

Figure B-2 Inactive channel

B.2 Load-type Blocks Load blocks apply the sample onto the column. There are two

modes that can be selected in the Method file— Pump or Manual

injection.

Pump injections draw liquid sample from the Sample In port and

routes it to the column (Figure B-3). The Mode valve should be in

the Operate position; the Effluent valve can either collect the

fluid or divert it to the channel’s Waste port.

Figure B-3 Fluid path for Load-type blocks -

Pump injections

Channel

Pump

Column

Sample

Channel Mode

Effluent

Pump

To Fox y

Sample In

Column

Contents

Main Page Foreword Page Page DANGER Hazard Symbols Page vii Tab le of Con ten ts Section 1 Introduction Section 2 Installation Section 3 Operation BioOptix 10 Table of Contents Section 4 System Maintenance Appendix A Method Templates Appendix B Fluid Path Diagrams Appendix C Replacement Parts Lists Table of Contents List of Figures List of Tables Section 1 Introduction 1.1 Product Overview Section 1 Introduction 1-2 1.2 System Specifications Table 1-1 BioOptix 10 Specifications 1.3 System Controls and Connectors Table 1-2 BioOptix 10 Front Panel Controls Table 1-3 BioOptix 10 Left-side Connections Table 1-4 BioOptix 10 Right-side Connections Table 1-5 BioOptix 10 Rear Panel Controls and Connections Section 2 Installation 2.1 Unpacking the BioOptix 10 System 2.2 BioOptix 10 Installation Page Page Page Page Page Page Page Page Page Page Table 2-1 Tubing Color chart Page 2.3 BioOptix 10 Software Installation Table 2-2 Windows PC Minimum Requirements 2.4 BioOptix 10 System Preparation Page Page Page Section 3 Operation 3.1 Sample Preparation 3.2 Column Installation 3.3 BioOptix 10 Software 3.4 Gradients 3.5 Methods Page Page Page 3.6 Experiments Page 3.7 Running the Experiment Table 3-1 Operation Buttons Page 3.8 Post-experiment Operation Page Page Page Section 4 System Maintenance 4.1 Periodic Maintenance 4.2 Exterior Cleaning 4.3 Piston Seal Replacement Page Section 4 System Maintenance 4-4 Figure 4-3 Pump assembly components Figure 4-4 Attach seal to push rod 4.4 Deuterium Lamp Replacement Page 4.5 Troubleshooting Table 4-1 Common Problems Page Page 4.6 Service Department 4.7 How to Ship Returns Appendix A Method Templates A.1 Opening the Template Page A.2 Modifying the Template Page Appendix B Fluid Path Diagrams B.1 Fluid-type Blocks B.2 Load-type Blocks B.3 Gradient-type Blocks B.4 Pump Flush B.5 Plumbing Diagram Page Page Appendix C Replacement Parts Lists Page Page Page Page Page Page Page Page Page Page Page Page Page CE Declaration of Conformity DECLARATION OF CONFORMITY Page ( Fax: Email: Teledyne Isco One Year Limited Factory Service Warranty *