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BioOptix™ 10 |
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Section 3 | Operation |
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3.5.3 | Wash Settings | This block flushes compounds that did not bind to the affinity | ||
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| media. The Wash tab is a fluid block type, therefore the settings | ||
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| displayed on this tab are the same as the Equilibrate tab. Refer | ||
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| to section 3.5.1 for a discussion on the settings for this tab. | ||
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Figure |
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3.5.4 Elute Settings | This block pumps the buffer solutions through the column. The | ||
| mixture and quantity of buffer solution is defined by the gradient | ||
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Figure 3-5 Elute tab settings
The Elute tab (Figure
•Gradient – Click the Browse button to select a gradient file.
•Volume, mL – This is reported by the gradient file.
•Flow Rate mL/min – type or select the desired flow rate.