BioOptix™ 10

 

 

 

Section 3

Operation

 

 

 

 

 

 

 

 

3.5.3

Wash Settings

This block flushes compounds that did not bind to the affinity

 

 

media. The Wash tab is a fluid block type, therefore the settings

 

 

displayed on this tab are the same as the Equilibrate tab. Refer

 

 

to section 3.5.1 for a discussion on the settings for this tab.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Figure 3-4 Wash tab settings

 

 

 

3.5.4 Elute Settings

This block pumps the buffer solutions through the column. The

 

mixture and quantity of buffer solution is defined by the gradient

 

file.

 

 

 

 

 

 

 

 

 

 

 

 

Figure 3-5 Elute tab settings

The Elute tab (Figure 3-5) contains the following:

Gradient – Click the Browse button to select a gradient file.

Volume, mL – This is reported by the gradient file.

Flow Rate mL/min – type or select the desired flow rate.

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Image 42
Teledyne 10 manual Wash Settings