BioOptix™ 10

Section 3 Operation

Figure 3-9 Pause at end of block

If the block is set to collect effluent (Figure 3-4), the fraction col- lector will deposit the fluid in the collection tubes. If a peak is detected, the fraction collector automatically advances to the next row to isolate the fluid. The fraction collector will also auto- matically advance to the next row of tubes whenever the spec- ified fraction size has been collected.

If the block is not set to collect effluent (Figure 3-2), the

BioOptix 10 diverts all pumped fluids to the waste ports.

3.7.2Operation Displays The window displayed during the run has several features that let you monitor the operation.

 

At the start of a run, the plot area displays the gradient as a blue

 

line (right Y-axis). The X-axis displays the volume pumped.

 

Below the X-axis is a progress bar. As the run progresses, the

 

absorbance traces is plotted as a red line (left Y-axis).

Zoom and pan

To the right of the plot area are several controls that can modify

 

the display. The Pan/Zoom switch specifies which action to take

 

when you use the cursor to click and drag across the plot area. In

 

Zoom mode, clicking and dragging across part of the plot area

 

will zoom in on the selected region. In Pan mode, clicking and

 

dragging moves the plot area under the cursor to a new position.

 

The view can be returned to the default view by clicking the

 

Reset Graph button.

Absorbance trace

In addition to the zoom feature which scales the plot area both

 

horizontally and vertically, the scale of the absorbance trace can

 

be modified alone. To change the left Y-axis Scale, click and select

 

a new value from the pull-down list.

Channel positions

Channel positions can be displayed in the plot area individually

 

or collectively. Highlight the channels you wish to display. If mul-

 

tiple absorbance traces and gradients are selected, they will be

 

overlaid in the plot area. This can be useful when comparing

 

channels, especially when desiring to monitor the effects of any

 

experimental interventions.

Status bar

The status bar is located at the bottom edge of the BioOptix 10

 

window. The fields in this bar display system information and

 

messages during the run.

3.7.3Suspending System The system automatically stops after completing all blocks.

Operation

However, the experiment can be paused at any time by clicking

 

the Stop button. This stops the pumps which allows you to refill

 

or change buffers, or make other mechanical adjustments to the

 

system. Click the Play button to resume the experiment.

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Teledyne 10 manual Zoom and pan, Operation