Teledyne 10 Section 4 System Maintenance

4-1

BioOptix™ 10

Section 4 System Maintenance

4.1 Periodic Maintenance The BioOptix 10 system’s internal components have been

selected for long life and low maintenance. To maintain the

system, the only required periodic maintenance is to flush the

pumps daily. The pump flush passes a wash fluid through the

pumps to prevent salt buffers from crystallizing in the system.

CAUTION

Salts will cause premature wear to the internal components

and eventually equipment failure. Flush the pumps daily to pre-

vent damage.

The BioOptix 10 system provides the option to flush the pump at

the end of a purification run (see section 3.8). If the system will

remain idle for a few hours or even overnight, a pump flush fol-

lowing the run is recommended.

The pump flush can also be initiated from a menu command.

Daily Pump Flush 1. Select Tools>Edit Pump Flush and review the settings. Save

any changes you’ve made.

2. Ensure the Buffer In lines are place d in containers of wash

fluid.

Note

Select a wash fluid that is strong enough to dissolve salts. The

wash fluid must also be miscible with buffer solution remaining

in the system from the previous run.

3. Select the To ol s> Pum p F lu sh menu command. This opens

the BioOptix 10 flush window (Figure 4-1).

4. Ch eck the pump channels to be flushed. Then, click the

Start button.

Figure 4-1 Flush window

Contents

Main Page Foreword Page Page DANGER Hazard Symbols Page vii Tab le of Con ten ts Section 1 Introduction Section 2 Installation Section 3 Operation BioOptix 10 Table of Contents Section 4 System Maintenance Appendix A Method Templates Appendix B Fluid Path Diagrams Appendix C Replacement Parts Lists Table of Contents List of Figures List of Tables Section 1 Introduction 1.1 Product Overview Section 1 Introduction 1-2 1.2 System Specifications Table 1-1 BioOptix 10 Specifications 1.3 System Controls and Connectors Table 1-2 BioOptix 10 Front Panel Controls Table 1-3 BioOptix 10 Left-side Connections Table 1-4 BioOptix 10 Right-side Connections Table 1-5 BioOptix 10 Rear Panel Controls and Connections Section 2 Installation 2.1 Unpacking the BioOptix 10 System 2.2 BioOptix 10 Installation Page Page Page Page Page Page Page Page Page Page Table 2-1 Tubing Color chart Page 2.3 BioOptix 10 Software Installation Table 2-2 Windows PC Minimum Requirements 2.4 BioOptix 10 System Preparation Page Page Page Section 3 Operation 3.1 Sample Preparation 3.2 Column Installation 3.3 BioOptix 10 Software 3.4 Gradients 3.5 Methods Page Page Page 3.6 Experiments Page 3.7 Running the Experiment Table 3-1 Operation Buttons Page 3.8 Post-experiment Operation Page Page Page Section 4 System Maintenance 4.1 Periodic Maintenance 4.2 Exterior Cleaning 4.3 Piston Seal Replacement Page Section 4 System Maintenance 4-4 Figure 4-3 Pump assembly components Figure 4-4 Attach seal to push rod 4.4 Deuterium Lamp Replacement Page 4.5 Troubleshooting Table 4-1 Common Problems Page Page 4.6 Service Department 4.7 How to Ship Returns Appendix A Method Templates A.1 Opening the Template Page A.2 Modifying the Template Page Appendix B Fluid Path Diagrams B.1 Fluid-type Blocks B.2 Load-type Blocks B.3 Gradient-type Blocks B.4 Pump Flush B.5 Plumbing Diagram Page Page Appendix C Replacement Parts Lists Page Page Page Page Page Page Page Page Page Page Page Page Page CE Declaration of Conformity DECLARATION OF CONFORMITY Page ( Fax: Email: Teledyne Isco One Year Limited Factory Service Warranty *