Technical Data

Hydraulics

Appendix C

Hydraulics

Dilution Ratio

When using the dual syringe model equipped with a 25 mL syringe on the left and a 100 µL syringe on the right, the dilution ratio is 1/25000, but in order to stay within the RSD range a maximum ratio of 1/500 is recommended.

Minimum Volume

The minimum programmable transfer volume is 1 µL. However, precise and accurate transfer of such volumes depends on the following parameters:

•volumetric capacity of the syringe,

•size of the air gap used,

•geometry of the needle tip,

•flow rate used,

•nature of the liquid to be transferred.

Maximum Volume

The maximum volume that can be aspirated from a vial is limited to the capacity of the syringe or on the transfer tubing, whichever is the smaller. The total aspirated volume (air gaps plus liquid volumes) must be inferior to this volume.

Flow Rate

Flow rates depend on the nature of the liquid (viscosity), the capacity of the syringe, and the internal diameter of tubing. For liquids transferred via the probe, it is possible to adjust the aspirate and dispense flow rates according to the nature of the liquid (e.g. viscous liquids must be aspirated slowly). The default value for ‘Air flow rate’ is 4 mL/min. Wathever the capacity of the syringe(s).

Appendix C-2

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Gilson 402 manual Hydraulics
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402 specifications

The Gilson 402 is a highly regarded laboratory device known for its precision and reliability in liquid handling applications. Primarily utilized in research and clinical labs, this automated pipetting system significantly enhances productivity by reducing the time taken for repetitive tasks while ensuring maximum accuracy.

One of the standout features of the Gilson 402 is its intuitive user interface. The touchscreen display allows operators to easily navigate through various functions and settings, enabling quick setup and adjustments. Its intuitive design minimizes the learning curve for new users, making it accessible even for those with limited technical expertise.

The Gilson 402 employs advanced pipetting technologies, which provide exceptional reproducibility and reliability. The system is equipped with proprietary Pipette G (as used in Gilson's PIPETMAN series), which ensures precise volume delivery. This is crucial in applications such as molecular biology, where even minor discrepancies in liquid volumes can lead to significant variations in experimental outcomes.

Another key characteristic of the Gilson 402 is its versatility. The device accommodates a wide range of pipette tips, allowing users to work with varying volumes and types of samples. Whether handling delicate biological fluids or viscous solutions, the Gilson 402 adapts to different protocols seamlessly.

The automation capabilities extend to a customizable programming feature that permits users to create complex pipetting protocols with ease. Multiple-step pipetting tasks can be completed effortlessly, reducing the likelihood of errors caused by manual handling.

Equipped with high-precision motors and sensors, the Gilson 402 also features built-in calibration and quality control functions. This guarantees that the system remains accurate over time and can easily be adjusted to maintain compliance with stringent laboratory standards.

In summary, the Gilson 402 is a cutting-edge liquid handling solution that combines advanced technology with user-friendly features. Its reliability, precision, and versatility make it an indispensable tool in modern laboratories. Whether used for routine tasks or complex experiments, the Gilson 402 enhances efficiency, promotes accuracy, and ultimately supports groundbreaking scientific research.
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