Gilson 402 manual Pipette, Operation - Advanced Use, Software, Key to press, air gap, fraction

Operation - Advanced Use 5

Press ESC (twice). Press ESC.

Pipette

Software

You use this task to aspirate a series of fractions via the probe, then dispense the total volume aspirated into a recipient vessel. Each liquid aspirated is sepa- rated by an air gap (if air gap is configured as > 0).

You may also aspirate an ‘Extra volume’ to ensure the accuracy of the first fraction. This ‘Extra volume’, which is aspirated with the first fraction, is dispensed at the end of the process, into the waste.

When you configure disposable tips in air mode, ‘Extra volume’ is replaced by ‘Air push volume’ in this task.

1.Aspirate an air gap.

2.Aspirate the first fraction (with ‘Extra volume’, if specified).

3.Aspirate an air gap.

4.Aspirate the second fraction.

5.Aspirate an air gap.

6.Aspirate the third fraction.

7.Dispense all except ‘Extra volume’ into recipient vessel.

8.Dispense ‘Extra volume’ to waste.

Key to press

1	2
air gap
fraction 1
		3
		air gap		4			7
			fraction 2			5	result
					air gap
					6
						fraction 3

Key in the File # (e.g. 45), ENTER Press Edit,

Press Pip, then select one of two modes, where:

(a)Fractions are equal,

(b)Fractions are different.

Press Chge to switch modes. Press ENTER, then Next.

Key in number of fractions,

press ENTER, then Next.

For SAME mode, key in common volume, for DIFFERENT mode, key in volume for fraction 1, press Next, key in volume for fraction 2, Next, etc.

Press ENTER, then Next.

5-25