Gilson 402 manual Syringe Correction Screen, Air Flow Screen Resv Flow Screen Prime Flow Screen

Operation - Advanced Use 5

Software

Syringe Correction Screen

When you turn the syringe correction factor ON, a plus sign

(+)appears after the file number, as a reminder.

Air Flow Screen

Resv Flow Screen

Prime Flow Screen

When you specify left and right syringe capacities (left must be > right), you must enter compatible volumes. An error message is displayed if you try to enter an incompatible volume, in which case you may need to change the volume of the other syringe, before entering the volume of the one that provoked the error message.

•Correction Factor

To compensate for the effects of temperature and viscosity, a factor may be applied to all liquid volumes. This factor should be calculated from gravimetric assays (see page 5-33).

With the Dual-syringe model, you must first select the syringe to which the correction applies. Starting from the Configuration Screen, press Syr, then use Next (or Prev) to select the right-hand or left-hand syringe.

Press Corr, you then see the Syringe Correction Screen. Key in a factor between 0 and 9.9 (%), press ENTER to confirm. Press ESC, then use Prev (or Next) to select the other syringe (if fitted), enter the correction in the same way; press ESC (twice) to return to the Configuration Screen.

Flow Rates

Press the Flow soft-key; the Air Flow Screen appears.

Air Flow is the rate in ml/min. that air is drawn into the transfer tubing when aspirating air.

Key in the required value, then press ENTER. Or, press Deflt to use the default value (4 mL/min).

Press the ESC key to return to the Configuration Screen or press Next to go to the Resv Flow Screen.

‘Resv asp:’ is the rate in mL/min. that solvent is aspirated from the reservoir by the left syringe.

Key in the required value, then press ENTER. Or, press Deflt to use the default value. Press the ESC key to return to the Configuration Screen or press Next to go to the Prime Flow Screen.

Prime Flow is the rate in mL/min. that liquid is transferred by the syringe(s) when priming.

Key in the required value, then press ENTER. Or, press Deflt to use the default value. Press the ESC key to return to the Configuration Screen. The flow- rate limits depend on the volume of the syringe(s) and type of tubing used, see pages 5-31 and 5-32.

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