Operation - Advanced Use

5

 

 

 

Software

Optionally, you can supply up to 12 reagents, press Reag, key in number of reagents, press ENTER.

Press Next, then for each reagent, key in volume press ENTER. After the data are entered the Reag key changes to REAG.

Press ESC until you see this screen (three times if you have added reagents)

Press ESC again to reveal the Save Screen; select Yes, the Method is then saved, after which you see the Run Screen.

Run Procedure

Press Run

Select the syringe (for the Dual-syringe 402 only), press the Chge soft-key to select LEFT or RIGHT, press ENTER to confirm.

You are advised to select the left-hand syringe if the volume to be measured seems to be significantly larger than the volume of the right-hand syringe.

The maximum volume that you can measure is limited to the volume of the aspirating syringe or the transfer tubing size, whichever is the smaller.

Press Next,

Key in the flow rate at which you want the volume measurement to take place, and then press ENTER. The default flow rate is half the maximum possible for the combination of syringe and transfer in use (refer to pages 5-31, 5-32).

Press Run, press the command button, aspirate the air gap:

Insert the tip of the tubing into the liquid to be measured press the command button once to start the measurement and a second time to stop. Refer to page 5-12 for details of the measuring process. At the end, the measured volume is shown on the screen.

At his point you can either aspirate diluent from the reservoir or via the probe. When you aspirate from the reservoir, the diluent volume and the measured volume are chained together in the transfer tubing and are dispensed together. If you use the probe, or if reagents are added, the liquids are dispensed in three steps:

(i)you dispense the measured sample,

(ii)you aspirate the diluent, reagents, and mea- sured sample,

(iii)you dispense all of the above into a vial.

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Gilson 402 manual Run Procedure
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402 specifications

The Gilson 402 is a highly regarded laboratory device known for its precision and reliability in liquid handling applications. Primarily utilized in research and clinical labs, this automated pipetting system significantly enhances productivity by reducing the time taken for repetitive tasks while ensuring maximum accuracy.

One of the standout features of the Gilson 402 is its intuitive user interface. The touchscreen display allows operators to easily navigate through various functions and settings, enabling quick setup and adjustments. Its intuitive design minimizes the learning curve for new users, making it accessible even for those with limited technical expertise.

The Gilson 402 employs advanced pipetting technologies, which provide exceptional reproducibility and reliability. The system is equipped with proprietary Pipette G (as used in Gilson's PIPETMAN series), which ensures precise volume delivery. This is crucial in applications such as molecular biology, where even minor discrepancies in liquid volumes can lead to significant variations in experimental outcomes.

Another key characteristic of the Gilson 402 is its versatility. The device accommodates a wide range of pipette tips, allowing users to work with varying volumes and types of samples. Whether handling delicate biological fluids or viscous solutions, the Gilson 402 adapts to different protocols seamlessly.

The automation capabilities extend to a customizable programming feature that permits users to create complex pipetting protocols with ease. Multiple-step pipetting tasks can be completed effortlessly, reducing the likelihood of errors caused by manual handling.

Equipped with high-precision motors and sensors, the Gilson 402 also features built-in calibration and quality control functions. This guarantees that the system remains accurate over time and can easily be adjusted to maintain compliance with stringent laboratory standards.

In summary, the Gilson 402 is a cutting-edge liquid handling solution that combines advanced technology with user-friendly features. Its reliability, precision, and versatility make it an indispensable tool in modern laboratories. Whether used for routine tasks or complex experiments, the Gilson 402 enhances efficiency, promotes accuracy, and ultimately supports groundbreaking scientific research.
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