Gilson 402 manual Run Procedure, Operation - Advanced Use, Software

Operation - Advanced Use 5

Software

Optionally, you can supply up to 12 reagents, press Reag, key in number of reagents, press ENTER.

Press Next, then for each reagent, key in volume press ENTER. After the data are entered the Reag key changes to REAG.

Press ESC until you see this screen (three times if you have added reagents)

Press ESC again to reveal the Save Screen; select Yes, the Method is then saved, after which you see the Run Screen.

Run Procedure

Press Run

Select the syringe (for the Dual-syringe 402 only), press the Chge soft-key to select LEFT or RIGHT, press ENTER to confirm.

You are advised to select the left-hand syringe if the volume to be measured seems to be significantly larger than the volume of the right-hand syringe.

The maximum volume that you can measure is limited to the volume of the aspirating syringe or the transfer tubing size, whichever is the smaller.

Press Next,

Key in the flow rate at which you want the volume measurement to take place, and then press ENTER. The default flow rate is half the maximum possible for the combination of syringe and transfer in use (refer to pages 5-31, 5-32).

Press Run, press the command button, aspirate the air gap:

Insert the tip of the tubing into the liquid to be measured press the command button once to start the measurement and a second time to stop. Refer to page 5-12 for details of the measuring process. At the end, the measured volume is shown on the screen.

At his point you can either aspirate diluent from the reservoir or via the probe. When you aspirate from the reservoir, the diluent volume and the measured volume are chained together in the transfer tubing and are dispensed together. If you use the probe, or if reagents are added, the liquids are dispensed in three steps:

(i)you dispense the measured sample,

(ii)you aspirate the diluent, reagents, and mea- sured sample,

(iii)you dispense all of the above into a vial.

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