Gilson manual Prime the 402 Man, Pressing End, Pressing STOP, Operation - Standard Use

Operation - Standard Use 4

Essential Steps

Manipulation Screen

Initialization Screen 2

Abort Screen

Prime the 402 (Man)

Prime is used to thoroughly clean out all fluid pathways with fresh solvent and to clear bubbles from the tubings.

Press the Man soft-key, the Manipulation Screen appears.

Press Prime, Initialization Screen 2 appears.

[Measure and Titrate are described in Chapter 5.]

You should prime the 402 before each run, to ensure that all fluid pathways contain fresh solvent and no air bubbles.

Put the probe-end of the transfer-tubing into a suitable the waste container, then press OK.

Priming starts immediately and the message ‘Priming ...’ appears on the screen; during which time solvent is expelled at the probe-end of the transfer tubing.

Press STOP, or the End soft-key, when you are satisfied that all fluid pathways have been primed with fresh solvent and are free of air bubbles.

Pressing End

When you press End, the motion of the syringe(s) ceases and ‘Homing ...’ appears on the screen until the syringe(s) have returned to their uppermost positions.

Pressing STOP

You can press STOP at any time, however, this key is more for use if a problem arises (such as a leak).

When you press STOP, the motion of the syringe(s) ceases and the Abort Screen appears. Press Proceed, when you want to continue the prime from the point at which it was stopped (for example, to refill the solvent reservoir).

Press Abort, when you want to stop priming; Initialization Screen 2 reappears. Ensure that the probe-end of the transfer-tubing is in a suitable waste container, then press OK.

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