Gilson 402 manual Additional Information, Flow Rates, Configuration, Methods Tasks

Operation - Advanced Use 5

Additional Information

Flow Rates

Flow rates depend on the nature of the liquid (viscosity), the capacity of the syringe, and the internal diameter of tubing. For liquids transferred via the probe, it is possible to adjust the aspirate and dispense flow rates according to the nature of the liquid (e.g. viscous liquids must be aspirated slowly). The default value for ‘Air flow rate’ is 4 ml/min. Whatever the capacity of the syringe(s).

In Configuration

In the configuration menus, you can modify the air flow rate and the flow rates of liquids coming from the Reservoir (Prime flow rate and Reservoir aspirate flow rate).

Configuration Flow Rates

Syringe size	Flow rate
	(mL/min)
100 µL	4
250 µL	10
500 µL	20
1 mL	40
5 mL	120
10 mL	120
25 mL	120

Additional Information

In Methods (Tasks)

In the Flow menu of each task, you can adjust the flow rates of liquid aspirated via the probe. Maxi- mum values (see tables) are given according to syringe capacity and internal diameter of the tubing. These maximum values have been opti- mized without air gap. When using air gap, it is recommended that you decrease these values.

Flow rates may be optimized using in-run prompts: when you enter a question mark in the place of a value (in task edit), that value has to be validated before the run. Optimization of flow rates is essential to assure maximum rapidity and volu- metric precision. The default values used by the software are shown in bold in the tables that follows.

When using Titrate or Measure, you are advised to

halve the maximum values shown in the tables. Maximum Aspirate Flow Rate (No Air Gaps)

Tubing Type			Syringe Volume (µL)
	100	250	500	1000	5000	10000	25000
1 ml	4	10	20	40	120	120	120
2 ml	4	10	20	40	100	100	100
5 ml	4	10	20	40	80	80	80
10ml	4	10	20	30	30	30	30
Micro Tips (liquid)	4	10	20	40	120	120	120
Micro Tips (air)	4	10	20	40	120	120	120
Large Tips (air)	4	10	20	40	120	120	120

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