Gilson 402 manual Technical Data Appendix C Syringes, Performance Specifications, Protocol

Technical Data Appendix C

Syringes

Syringe capacity (µL)	100	250	500	1000	5000	10000	25000
Maximum Flow (mL/min)	4	10	20	40	120	120	120
Minimum Specified Volume (µL)	10	25	50	100	500	1000	2500
Nominal Volume (µL)	100	250	500	1000	5000	10000	25000

Hydraulics

Performance Specifications

The following values are obtained using a defined protocol. If this protocol is followed, the obtained values must be inferior or equal to the listed figures.

The values are obtained by pipetting de-ionized water (grade 3, ISO3696) at 21 °C ± 0.5 °C, relative humidity > 50 % and athmospheric pressure 1013 MPa.

	Syringe	Volume Systematic error Random error
	capacity (µL)	pipetted (µL)	%	µL	%	µL
		10	1.8	0.18	0.8	0.08
100		50	1.5	0.75	0.25	0.125
		100	1	1	0.15	0.15
		20	1.5	0.38	0.8	0.2
250		125	1.0	1.25	0.25	0.32
		250	0.8	2	0.15	0.38
		50	1.5	0.75	0.50	0.25
500		250	1.0	2.5	0.25	0.63
		500	0.8	4	0.15	0.75
		100	1.5	1.5	0.50	0.5
1000		500	0.8	4	0.25	1.3
		1000	0.6	6	0.15	1.5
		500	1.2	6	0.5	2.5
5000		2500	0.8	20	0.25	6.3
		5000	0.6	30	0.15	7.5
		1000	1.2	12	0.5	5
10000		5000	0.8	40	0.25	12.5
		10000	0.5	50	0.15	15
		2000	1.0	25	0.50	5
25000		12500*	0.8	100	0.25	31.3 * Dispensing mode only.
		25000*	0.5	125	0.15	37.5

Protocol

Configuration: 1 mL tubing for volumes up to 1 mL, tapered transfer-tubing of 2 mL (standard accessory) for volumes up to 2 mL, 5 mL or 10 mL tapered transfer tubings are used for volumes up to 10 mL.

Transfer of de-ionized water using the Pipette Task, aspiration of liquid from a vial and delivery at the same flow rate into another vial containing water.

Appendix C-3