Gilson 402 manual Screens Key to press, Press Chge to select source, press ENTER, then Next

Operation - Advanced Use 5

Software

For each dilution in the series: Vt = Vs x Rd, Vd = Vt - Vs

For example, after selecting Dilution Ratio Mode, you enter Sample Volume = 10 and Dilution Ratio = 3; the software calculates the Total Volume as 30 and the required Diluent Volume as 20.

1. When diluent comes from the reservoir (RESV);

		aspirate the required volume of diluent (for all
		dilutions, up to the capacity of the left-hand
		syringe). If diluent comes from the probe, an air
		gap is aspirated before the diluent, and the
		required volume of diluent is aspirated (for all
		dilutions, up to the capacity of the right-hand
		syringe, after which the left-hand syringe is used).
	2.	Aspirate an air gap (specified in configuration).
	3.	Aspirate sample.
	4.	Dispense all into the recipient vessel.
	5.	Aspirate an air gap (specified in configuration).
	6.	Aspirate diluted sample.
	7.	Dispense all into the recipient vessel.
	8.	Aspirate an air gap (specified in configuration).
	9.	Aspirate diluted sample.
	10. Dispense all into the recipient vessel.
	11. And so on ...
	Typical Task Entry
Screens:	Key to press:
	Key in the File # (e.g. 48),
	Press ENTER
	Press Edit
		Press Dil
		Press Serial

Press Chge to select source, press ENTER, then Next.

Press Chge to select mode, press ENTER,

Press Next, key in a number of serial dilutions, press ENTER.

Press Next, then key in the sample volume, press

ENTER.

Depending on the mode, you see one of three screens after pressing Next.

Key in the value, press ENTER.

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