Operation - Advanced Use

Syringe Correction Function

A correction factor may be applied to all volumes of liquid to compensate for the effects of temperature (for example, when the 402 is used in a culture room) or for the effects of viscosity. This factor should be calculated from gravimetric assays and entered in the Configuration menu. With the Dual-syringe model, you may apply different corrections to each syringe.

When the syringe correction is switched on, a plus sign (+) appears after the Method number. All volumes in all the tasks are affected by this correc- tion, when it is switched on. At the start of a task edit, a message appears to inform you of the status (Syringe Correction Screen, see page 5-8).

A correction factor of 1% (for example) under the same condition of temperature and pressure in- creases the volume of liquid aspirated or dispensed by this amount.

The correction allows you to increase the volume transferred, for example: 250 µL syringe, nominal value is 100 µL, the actual value (after gravimetric measurements) is 99 µL. In this case, a 1% correction results in a nominal value of 101 µL, and an actual value of 100 µL. The application of a 1 % correction factor effectively reduces the capacity of the syringe by 1 %.

You should follow the procedure for gravimetric analysis described in the Operational Qualification (OQ) document that accompanies this User’s Guide. Check the accuracy and RSD of the volumes with the liquid to be transferred under the conditions of pressure and temperature of the working environment.

Use of Disposable Tips

Using the disposable tip kit, Gilson D200 tips or Gilson D1000 tips can be mounted at the end of the probe. Two modes are available: air and liquid.

Air Mode

The use of 402 in ‘Air Mode’ is, for those users familiar with Gilson’s pipettes, the same as that of ‘Pipetman’.

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Additional Information

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Gilson 402 manual Operation Advanced Use Syringe Correction Function, Use of Disposable Tips, Air Mode

402 specifications

The Gilson 402 is a highly regarded laboratory device known for its precision and reliability in liquid handling applications. Primarily utilized in research and clinical labs, this automated pipetting system significantly enhances productivity by reducing the time taken for repetitive tasks while ensuring maximum accuracy.

One of the standout features of the Gilson 402 is its intuitive user interface. The touchscreen display allows operators to easily navigate through various functions and settings, enabling quick setup and adjustments. Its intuitive design minimizes the learning curve for new users, making it accessible even for those with limited technical expertise.

The Gilson 402 employs advanced pipetting technologies, which provide exceptional reproducibility and reliability. The system is equipped with proprietary Pipette G (as used in Gilson's PIPETMAN series), which ensures precise volume delivery. This is crucial in applications such as molecular biology, where even minor discrepancies in liquid volumes can lead to significant variations in experimental outcomes.

Another key characteristic of the Gilson 402 is its versatility. The device accommodates a wide range of pipette tips, allowing users to work with varying volumes and types of samples. Whether handling delicate biological fluids or viscous solutions, the Gilson 402 adapts to different protocols seamlessly.

The automation capabilities extend to a customizable programming feature that permits users to create complex pipetting protocols with ease. Multiple-step pipetting tasks can be completed effortlessly, reducing the likelihood of errors caused by manual handling.

Equipped with high-precision motors and sensors, the Gilson 402 also features built-in calibration and quality control functions. This guarantees that the system remains accurate over time and can easily be adjusted to maintain compliance with stringent laboratory standards.

In summary, the Gilson 402 is a cutting-edge liquid handling solution that combines advanced technology with user-friendly features. Its reliability, precision, and versatility make it an indispensable tool in modern laboratories. Whether used for routine tasks or complex experiments, the Gilson 402 enhances efficiency, promotes accuracy, and ultimately supports groundbreaking scientific research.