Gilson 402 Operation - Advanced Use Syringe Correction Function, Use of Disposable Tips, Air Mode

Operation - Advanced Use

Syringe Correction Function

A correction factor may be applied to all volumes of liquid to compensate for the effects of temperature (for example, when the 402 is used in a culture room) or for the effects of viscosity. This factor should be calculated from gravimetric assays and entered in the Configuration menu. With the Dual-syringe model, you may apply different corrections to each syringe.

When the syringe correction is switched on, a plus sign (+) appears after the Method number. All volumes in all the tasks are affected by this correc- tion, when it is switched on. At the start of a task edit, a message appears to inform you of the status (Syringe Correction Screen, see page 5-8).

A correction factor of 1% (for example) under the same condition of temperature and pressure in- creases the volume of liquid aspirated or dispensed by this amount.

The correction allows you to increase the volume transferred, for example: 250 µL syringe, nominal value is 100 µL, the actual value (after gravimetric measurements) is 99 µL. In this case, a 1% correction results in a nominal value of 101 µL, and an actual value of 100 µL. The application of a 1 % correction factor effectively reduces the capacity of the syringe by 1 %.

You should follow the procedure for gravimetric analysis described in the Operational Qualification (OQ) document that accompanies this User’s Guide. Check the accuracy and RSD of the volumes with the liquid to be transferred under the conditions of pressure and temperature of the working environment.

Use of Disposable Tips

Using the disposable tip kit, Gilson D200 tips or Gilson D1000 tips can be mounted at the end of the probe. Two modes are available: air and liquid.

Air Mode

The use of 402 in ‘Air Mode’ is, for those users familiar with Gilson’s pipettes, the same as that of ‘Pipetman’.

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Additional Information

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